The aim of this thesis is to study cell-cell interactions and the development of an assay to explore and quantify the exchange of membrane compounds.

The way organisms develop from the initial single-cellular state to a complex final assembly like the human body, and how the final body is maintained throughout life, is one of the greatest mysteries and it’s understanding one of the biggest scientific challenges.

Despite being the object of intense study, embryonic development has been difficult to model due to a number of reasons. First, complex tissues can be comprised of many cell types, of which we probably only know a subset.

The single cell group (SCG) is part of the Metabolomics & Analytics Centre Our research is centered around developing innovating analytical methods tailored for small-volume, or single cell analysis to enable metabolomics-driven systems biology in personalized health strategies. We are always on…

Synthetic or artificial cells are vesicles, often lipid vesicles, with wide ranging applications from drug delivery to nanoreactors in biocatalysis. We are developing polymer-lipid hybrid vesicles for applications in compartmentalised biocatalysis, in which the polymer has been shown to greatly enhance…

Atherosclerosis is the most prominent underlying pathology of cardiovascular disease and an important cause of major adverse cardiovascular events.

Leiden has a long history in the treatment of blood cell cancer. Research to find better therapies never stands still. One of the potential treatments currently being worked on is a ‘living medicine’.

Promotor: Prof.dr. J. Kuiper, Co-Promotor: S.C.A. de Jager

This book explores the lasting legacy of the controversial project by the Congress for Cultural Freedom, funded by the CIA, to promote Western culture and liberal values in the battle of ideas with global Communism during the Cold War.

Despite the wealth of historical literature on the Second World War, the subject of religion and churches in occupied Europe has been undervalued.

In this thesis, we describe the potential of cell mechanical phenotyping for immune cell characterization, diagnosis, drug testing, as well as treatment of disease.

Mechanical interactions between cells and their environment play an important role in many biological processes.

The bacterial cell wall is a nearly universal structure that offers protection and gives the cell its shape. However, environmental stressors, such as cell wall-targeting antibiotics and hyperosmotic conditions, can induce bacteria to shift to a wall-deficient state. It is unknown whether the lack of…

Volume I of The Cambridge History of Strategy offers a history of the practice of strategy from the beginning of recorded history, to the late eighteenth century, from all parts of the world.

What distinguishes a tumour cell from a healthy cell? Researchers are trying to answer this question as precisely as possible. Certain differences could eventually lead to new therapies.

The CMCB brings together microbial cell biology expertise in Leiden. It aims at optimal resonance between the groups and maximal exploitation of the available infrastructure and to develop innovative cross-boundary technology platforms.

Cardiovascular disease is a major global burden and atherosclerosis is the main underlying pathological process.

Bas Rietjens and his colleagues researched the Russia-Ukraine war, exploring this multitude of facets and their interconnections.

Almost all bacteria are enveloped by a cell wall that provides cellular protection.

Promotor: R.C.M. Pelger Co-Promotor: G. van der Pluijm

Acute cardiovascular syndromes, including myocardial infarction or stroke, are the principal cause of death in the Western society. The main underlying pathology of cardiovascular diseases is atherosclerosis, which is caused by the accumulation of lipids and inflammatory cells in the vessel wall, in…

Cell-based medicinal products (CBMPs), such as CAR T cells, are highly complex biopharmaceutical products offering various treatment options for serious illnesses such as cancer or autoimmune diseases.

The research within the Division of Cell Systems and Drug Safety, headed by Prof. Bob van de Water, is focused on novel therapeutic modalities and novel concepts in early drug discovery, in order to develop more effective and safer therapeutic strategies. We generate advanced cell and computational…

Which brain cell does what, when Parkinson's disease arises? It won't be long before this jigsaw is solved piece by piece. Ahmed Mahfouz, computational biologist, combines bio-knowledge from Leiden with algorithms from Delft and is getting closer to finding the key.

Japan's Occupation of Java in the Second World War draws upon written and oral Japanese, Indonesian, Dutch and English-language sources to narrate the Japanese occupation of Java as a transnational intersection between two complex Asian societies, placing this narrative in a larger wartime context of…

The aim of this thesis was to study phage infection in Streptomyces, focused on cell wall-deficient cells. Bacteriophages (or phages in short) attach to the cell wall of bacteria after which they replicate and lyse the host cell.

Atherosclerosis is the most important underlying process that drives cardiovascular disease, and is characterized by an accumulation of cholesterol which triggers an inflammatory response in the vessel wall.

In this article, Jasmijn Boeken, explores in which ways the defining characteristics of the different zones can be found in the digital sphere.

Atherosclerosis, in particular rupture of atherosclerotic plaques, is the underlying cause of most heart attacks or strokes and is the leading cause of death worldwide.

Atherosclerosis is the main underlying pathology of cardiovascular disease. Atherosclerosis is caused by an immune response which is directed against (modified) lipoproteins which accumulate in the vessel wall.

Cardiovascular syndromes are the major cause of death in Western societies.

Source: Biosens Bioelectron, Volume 74, pp. 233-42 (2015)

From molecule, to cell, to organism. The high-level drug research at the Cell Observatory aimes at reducing the spread of cancer and developing new medicines for tuberculosis.

In this thesis, single-cell approaches were applied to characterize the impact of aging on the immunological B cell landscape in different models of atherosclerotic cardiovascular disease, with the goal to identify and study new B cell-associated biomarkers and therapeutic targets to halt atherosclerosis…

Epithelial-mesenchymal plasticity (EMP) and tumor cell migration play an important role in cancer progression, and an improved understanding of the mechanisms underlying these concepts is essential for developing new targeted approaches.

Treatment with special stem cells seems to be a good option for suppressing the immune system in organ transplants. Researchers are trying to learn from processes that take place in the human body during pregnancy.

Cell adhesion signaling

A new therapy for the serious Graft-versus-Host disease

In this article, Ben de Jong, research fellow at the Institute of Security and Global Affairs, examines the relationship between double agents and their handlers.

Japan's Occupation of Java in the Second World War draws upon written and oral Japanese, Indonesian, Dutch and English-language sources to narrate the Japanese occupation of Java as a transnational intersection between two complex Asian societies, placing this narrative in a larger wartime context of…

Mechanobiology is a major area in biology, however, mechanics is currently a missing element in drug development pipelines. This has led to a high cost for the society. Mechanotoxicity of drugs often appears as a long-term side effect of drugs, and has led to removal of drugs from the market. Furthermore,…

In this research project, we aimed to obtain more insight in the role of mast cells in the immune driven disorders rheumatoid arthritis and atherosclerosis.

This thesis advances our understanding of lipid uptake, a vital first step in lipid metabolism, by developing innovative click chemistry-based tools to study how immune cells internalize lipids.

The historiography of the Bretton Woods conference of July 1944 is dominated by the personal clash between the principal negotiators, Harry Dexter White of the United States and John Maynard Keynes of Britain.

There is a need for alternative methods to replace, reduce and refine (3R) animal experimentation. Combining experimental data from high-throughput in vitro studies with in silico modeling is a promising approach to unravel the effect of chemicals on living cells and to gain a better understanding of…

This work describes the use of click-to-release chemistry to get spatiotemporal control over immunocytokine activity. Until now, immunocytokines (cytokines coupled to a tumor-targeting-moiety) remained active throughout the body, being able to bind their respective receptors, causing mild to severe…