The aim of this thesis is to study cell-cell interactions and the development of an assay to explore and quantify the exchange of membrane compounds.

Despite being the object of intense study, embryonic development has been difficult to model due to a number of reasons. First, complex tissues can be comprised of many cell types, of which we probably only know a subset.

This dissertation focuses on investigating the public communication around quantum science and technology that may affect public engagement.

In this thesis, we describe the potential of cell mechanical phenotyping for immune cell characterization, diagnosis, drug testing, as well as treatment of disease.

Mechanical interactions between cells and their environment play an important role in many biological processes.

Promotor: Prof.dr. J. Kuiper

There is an urgent need for more physiologically relevant cell culture methods to guide compound selection in pre-clinical stages of the drug development pipeline.

The aim of this thesis was to study phage infection in Streptomyces, focused on cell wall-deficient cells. Bacteriophages (or phages in short) attach to the cell wall of bacteria after which they replicate and lyse the host cell.

Cardiovascular disease is a major global burden and atherosclerosis is the main underlying pathological process.

Cell-based medicinal products (CBMPs), such as CAR T cells, are highly complex biopharmaceutical products offering various treatment options for serious illnesses such as cancer or autoimmune diseases.

Epithelial-mesenchymal plasticity (EMP) and tumor cell migration play an important role in cancer progression, and an improved understanding of the mechanisms underlying these concepts is essential for developing new targeted approaches.

There is a need for alternative methods to replace, reduce and refine (3R) animal experimentation. Combining experimental data from high-throughput in vitro studies with in silico modeling is a promising approach to unravel the effect of chemicals on living cells and to gain a better understanding of…

This work describes the use of click-to-release chemistry to get spatiotemporal control over immunocytokine activity. Until now, immunocytokines (cytokines coupled to a tumor-targeting-moiety) remained active throughout the body, being able to bind their respective receptors, causing mild to severe…

Gaucher disease is a rare metabolic disorder, in which a mutation of the enzyme glucocerebrosidase (GBA1) causes buildup of its substrate glucosylceramide and the deacylated counterpart glucosylsphingosine in lysosomes.

This thesis advances our understanding of lipid uptake, a vital first step in lipid metabolism, by developing innovative click chemistry-based tools to study how immune cells internalize lipids.

In this thesis, single-cell approaches were applied to characterize the impact of aging on the immunological B cell landscape in different models of atherosclerotic cardiovascular disease, with the goal to identify and study new B cell-associated biomarkers and therapeutic targets to halt atherosclerosis…

A new type of tripodal squaramide-based supramolecular hydrogels is developed and studied.

During embryonic growth, cells proliferate, differentiate, and collectively migrate to form different tissues at the right position and time in the body.

Almost all bacteria are enveloped by a cell wall that provides cellular protection.

Aspergillus niger is an important industrial producer of organic acids and enzymes producing large amounts of spent fungal biomass.

In this project of the Center for Public Values & Ethics (CPVE) ongoing research is conducted on the history of advice to Dutch central government by so-called State committees from 1814 to 2014. State committees have been installed by Dutch central government since 1814 to provide expert advice, research…

This dissertation aims to advance understanding of amoeboid single cell migration under the influence of changing physicochemical environmental properties. The particular focus is on the relatively unknown influence of differing physical properties in the extracellular environment, such as the migratory…

Traditional drug discovery approaches have been hampered by (in vitro) cell-culture models that poorly represent the situation in the human body.

Acute cardiovascular syndromes, including myocardial infarction or stroke, are the principal cause of death in the Western society. The main underlying pathology of cardiovascular diseases is atherosclerosis, which is caused by the accumulation of lipids and inflammatory cells in the vessel wall, in…

In this research project, we aimed to obtain more insight in the role of mast cells in the immune driven disorders rheumatoid arthritis and atherosclerosis.

In this thesis, we have studied cell images from two types of cells, including pollen grains and the immune cells, neutrophils. These images are captured using a bright field microscope and a confocal microscope.

This thesis focuses on two processes involved in fighting infections: metabolism and immune cell motility and navigation.

Wil jij jouw eigen vragen en ideeën vertalen naar wetenschappelijke onderzoeksprojecten? Worden jouw interesses geprikkeld door technologie? Durf jij wetenschap kritisch te benaderen en haar grenzen te verleggen?

Waarom Molecular Science & Technology in Leiden? Je wordt niet chemicus óf technoloog, maar beide! De opleidingsvideo vertelt je er alles over.

Het beste van Leiden en Delft komt samen in de bachelor opleiding Life Science & Technology. Benieuwd naar het programma? Bekijk de video.

The bacterial cell wall is a nearly universal structure that offers protection and gives the cell its shape. However, environmental stressors, such as cell wall-targeting antibiotics and hyperosmotic conditions, can induce bacteria to shift to a wall-deficient state. It is unknown whether the lack of…

Deze tweejarige masteropleiding wordt aangeboden door het Leiden Institute of Chemistry en heeft een focus op technologische en fundamentele kennis en expertise. Tijdens de opleiding doe je onderzoek naar de processen van de levende cel met fundamenteel wetenschappelijke én toegepaste technologie. Je…

De onderzoeksgroep Business, Technology & Public Policy van de afdeling Bedrijfswetenschappen van Universiteit leiden houdt zich bezig met het onderzoeken van de dynamische wisselwerking tussen ‘nieuwe’ technologieën en de verschillende belanghebbenden die hiermee interacteren, waaronder consumenten,…

This dissertation is organised into three parts, each addressing a distinct research area related to Gibbs measures.

Quantum Information Science & Technology (QIST) encompasses the understanding, design, construction and investigation of quantum information processing systems, such as quantum computers, quantum communication networks, and quantum sensors.

The emergence of complex diseases resulting from abnormal cell-cell signaling and the spread of infectious diseases caused by pathogens are significant threats to humanity. Unraveling the dynamic mechanisms underlying cell-cell signaling and infectious disease spreading is crucial for effective disease…

Promotor: W.Th.F. den Hollander Co-Promotor: F.M. Spieksma

CONCLUSION: This study demonstrates that human macrophages can be induced to exert direct anti-tumor activity against osteosarcoma cells. Our observation that the induction of macrophage anti-tumor activity by L-MTP-PE required IFN-gamma may be of relevance for the optimization of L-MTP-PE therapy in…

The way organisms develop from the initial single-cellular state to a complex final assembly like the human body, and how the final body is maintained throughout life, is one of the greatest mysteries and it’s understanding one of the biggest scientific challenges.

Bij de bacheloropleiding Life Science and Technology leer je de biologie van de cel doorgronden, en leer je ingewikkelde problemen op een efficiënte en systematische manier oplossen.

DNA-profilering op een crime-scene, DNA-chips voor het opsporen van ziektegerelateerde genen, wasmiddelen met enzymen die bij hoge temperatuur nog werkzaam zijn, bacteriën die helpen bij het zuiveren van afvalwater en het gebruik van gentherapie als medicijn.

This thesis describes the design and synthesis of fragments of various cell wall carbohydrates of the Streptococcus species, including the branched Group B-specific antigen (GBC) of Group B Streptococcus, glycerol phosphate (GroP) modified group A carbohydrate (GAC), and the O-acetylated type 1 capsular…

This thesis examines strategies to address the global threat of antimicrobial resistance (AMR). Antimicrobial resistance poses an increasing global health risk, highlighting the need for new treatments against Gram-negative pathogens.

In order to gain a better insight into the basis of the rapid jasmonic acid (JA)-mediated stress response, an integrated approach using a targeted high-resolution mass spectrometry-based (HRMS) platform, was conducted to monitor the metabolism of JA using the model system of cell suspension cultures…

Onder de gezamenlijke titel ‘Scarcity and the State’ verschenen onlangs twee Engelstalige boeken over de verdeling van schaarse publieke rechten (Intersentia 2016). Paul Adriaanse (UL), Frank van Ommeren (VU), Willemien den Ouden (UL) en Johan Wolswinkel (Tilburg University) voerden de redactie. Aan…

Bij de bacheloropleiding Molecular Science & Technology aan de Universiteit Leiden ontwikkelen studenten een sterke theoretische basis in de scheikunde en doen ze kennis van de technologische toepassingen op. Daarnaast leren studenten een bijdrage te leveren aan vraagstukken op het gebied van duurzaamheid,…

Dit artikel onderzoekt hoe tijdelijke coalities van niet-statelijke actoren deelnemen aan internationale onderhandelingen over milieuvraagstukken.

Bacteria have the ability to alter their morphology in order to adapt to changing environments.

De lezingreeks Humanity in the Automated State Jaar 2025/2026 onderzoekt hoe AI en geautomatiseerde systemen de overheid en publieke administratie transformeren en wat het betekent om mens te zijn binnen deze gedigitaliseerde instituties.