Increasing the efficiency of gene targeting (GT) as a genome editing tool in plants has been an important goal in plant biotechnology.

De faciltieit ontwikkelt en gebruikt, high-throughputmethoden toe voor genomisch onderzoek.

In this thesis I highlight the applications of Oxford Nanopore Technologies (ONT) sequencing. This technique is a relatively new approach in the sequencing field, where nanopores are embedded in a membrane, DNA molecules are pulled through nanopores and an electrical current serving as the sequencing…

Parkinson's disease is the second most common neurodegenerative disease in the world. One of its symptoms is the loss of dopaminergic neurons in the substantia nigra pars compacta.

The four possible segments A, T, C and G that link together to form DNA molecules, and with their ordering encode genetic information, are not only different in name, but also in their physical and chemical properties.

Promotor: P.J.J. Hooykaas, Co-Promotor: E.J. van der Zaal

Every organisms in the tree of life faces the same challenge: the length of its DNA exceeds the volume of the cell it needs to fit in. Several strategies have evolved to solve this problem, one of them being the expression of proteins that bind and organize the DNA.

All life on earth contains DNA, which is used to store biological information. Organisms compact their DNA in order for it to fit inside their cell(s).

Climate change is a challenge for both current and future generations. New biological resources have to be developed in order to meet the demand for energy as well as the demand for food.

This thesis focuses on two processes involved in fighting infections: metabolism and immune cell motility and navigation.

The increasing resistance of pathogenic bacteria to antibiotics necessitates the discovery of new medicines.

Bacterial biocatalysis constitutes a sustainable alternative for high-value chemicals production by enabling the utilization of renewable feedstocks.

Fungal food spoilage often starts with a contamination with spores. Experimental data strongly indicate the existence of subpopulations of spores with different levels of resistance to preservation methods.

This thesis pioneers diatom molecular identification and quantification through genome-scale methods, with four key aims: (i) reviewing DNA/RNA sequencing methods in aquatic biomonitoring to highlight their strengths and limitations;

The ongoing increase in antimicrobial resistance combined with the low discovery of novel antibiotics is a serious threat to our health care.

Promotor: Jaap Brouwer, Co-promotor: Remus Th. Dame

Thirty years ago, mutations in the BRCA1 gene were first linked to hereditary breast and ovarian cancer, establishing a genetic basis for cancer development.

Promotie

Promotor: P. J. J. Hooykaas Co-promotor: B. S de Pater

Bacteria often experience external challenges, such as changes in environmental conditions or attacks by bacteriophages.

In this thesis, I investigated the neurogenetic properties of Neotropical bat species. Bats represent the second largest mammalian order (Chiroptera) in the world, with Neotropical bats constituting about 13% of the total number of bat species.

Promotie

The RNA guided endonuclease based on the CRISPR/Cas system of Streptococcus pyogenus is a potent new tool for genome engineering in plants.

This dissertation is part of a broader research project that revolves around unraveling the evolution of balanced lethal systems.

Promotor: M. Ubbink, Co-Promotor: R. T. Dame

Hybrid zones occur where two species meet and produce offspring (hybrids). Typically, hybrids show a considerable reduction in fitness. In this thesis two hybrid zones are treated.

Promotor: J.N. Kok, P.E. Slagboom Co-promotor: J.F.J. Laros

Streptomyces are filamentous bacteria that produce more than two-thirds of known antibiotics.

The investigations described in this thesis lay out strategies aimed at advancing antibiotic research and development. The examples presented revolve around two main approaches: understanding drug-target interactions and target identification.

All crested and marbled newts (the genus Triturus) suffer from an unusual genetic abnormality, called a balanced lethal system, that kills exactly half of their offspring. How can a trait so disadvantageous have survived millions of years of natural selection?

Understanding DNA repair mechanisms is essential for advancing plant biotechnology and crop improvement. This study investigates how double-strand breaks (DSBs), induced by CRISPR/Cas systems, are repaired in the model plant Arabidopsis thaliana, and how these processes influence Agrobacterium tumefaciens…

The objective of this study was to investigate the expression and function of GRHL2 in different breast cancer subtypes.

The human body consists of hundreds, perhaps thousands of different types of cells, each with different morphologies and functions, despite having the same genome.

The bacterial cell wall is a nearly universal structure that offers protection and gives the cell its shape. However, environmental stressors, such as cell wall-targeting antibiotics and hyperosmotic conditions, can induce bacteria to shift to a wall-deficient state. It is unknown whether the lack of…

In human cells, a meter-long DNA is condensed inside a micrometer-sized cell nucleus. Simultaneously, the genetic code must remain accessible for its replication and transcription to functional proteins.

The haplolepideous mosses (Dicranidae) comprise about 4000 species distributed over a wide range of habitats, with great gametophytic and sporophytic morphological variation. Their monophyly is well supported by the results of several molecular phylogenetic studies, which shed light on their relationships…

Supervisor: P.J.J. Hooykaas

Plant-microbe interaction resulted in different physio/chemical responses by host plant and interacting rhizobacteria.

Acidobacteria is a widely distributed phylum but their functional roles in ecosystem processes are still largely elusive.

The AT-Hook Nuclear Localized 15 (AHL15) gene belongs to a plant-specific gene family of DNA-binding proteins. Overexpression of AHL15 in Arabidopsis thaliana delays developmental phase transitions whereas its loss of function causes a slight acceleration.

Chapter 2 describes a post-synthetic methodology to introduce a fluorescent label in highly lipophilic, Pam3Cys based conjugates.

Promotie

Promotor: G.P. van Wezel, Co-promotor: Y.H. Choi

Wereldwijde uitdagingen aanpakken via internationale wetenschappelijke consortia

Promotie

Promotie

Het LUMC staat voor de verbetering van de gezondheidszorg en de gezondheid van mensen. Deze missie brengen de medewerkers van het LUMC dagelijks in de praktijk met toonaangevend onderzoek, vernieuwend onderwijs en optimale, innovatieve zorg.

Meer informatie over de organisatie van het CML is beschikbaar op de Engelse versie van de CML-website