Promotor: Prof.dr. H.S. Overkleeft

Science Based Business (SBB) verbindt de domeinen wetenschap, bedrijfsleven en beleid. Wetenschappelijk onderzoek waar we ons in Leiden op richten, bijvoorbeeld op het gebied van kunstmatige intelligentie, belooft onze samenlevingen ten goede te komen. Het vertalen van wetenschap naar positieve maatschappelijke…

Lysosomal acid glucosylceramidase (GBA1) is a lysosomal enzyme that degrades glucolipids with its main substrate being glucosylceramide (GlcCer). Defects in the GBA1 gene lead to glycosphingolipidosis Gaucher disease (GD), in which the hydrolysis of GlcCer is impaired and therefore, it accumulates in…

Supramolecular polymers are class of materials that are formed by non-covalent interactions such as hydrogen bonding, π-π interactions, electrostatic interactions and the hydrophobic effect.

In the era of big data, extracting insights from complex datasets is a key challenge. This thesis demonstrates the superiority of graph-based methods over traditional clustering (e.g., k-means, DBSCAN) and outlier detection for analyzing high-dimensional and noisy data.

Promotor: K.J. Batenburg

This thesis describes the use of an activity-based proteomics method to study the endocannabinoid system.

Promotor: Prof.dr. H.A.G. Wijshoff

The main goal of this thesis is to achieve advanced control of binding properties in bis-pyrrolic and macrocyclic pyrrolic receptors by light.

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt

Promotores: H.S. Overkleeft, G.A. van der Marel, Co-promotor: B.I. Florea

Neuraminidases are enzymes that cleave glycosidic linkages of sialic acid. These enzymes are involved in influenza infections as well as in many cellular processes in mammals and micro-organisms.

Microscopic insight on lipid-based nanomedicine in vivo remains limited to the perception of the knowledge that could be obtained: if we interpret only what we see, then we only believe to know. Although believing to know encapsulates an undefined amount of uncertainty in the exploration of lipid-based…

Glycosidases are important enzymes in the turnover of polysaccharides and glycoconjugates, and are involved in a range of human pathologies including genetic disorders such as Gaucher and Pompe disease, but also in various cancers.

In the last decades, activity-based protein profiling (ABPP) has emerged as a powerful chemical tool that may aid the ever-challenging drug discovery process.

Promotor: H.S. Overkleeft, J. Neefjes, Co-promotor: M. van der Stelt

Promotores: Prof.dr.G.R. de Snoo and Dr. R. Heijungs

Inverse problems are problems where we want to estimate the values of certain parameters of a system given observations of the system. Such problems occur in several areas of science and engineering. Inverse problems are often ill-posed, which means that the observations of the system do not uniquely…

Promotor: Prof.dr. J.G.E.M. Fraaije, Co-promotor: A. Kros

Business Process Model and Notation (BPMN) has become the standard for business processes diagrams.

The aim of this thesis was to develop aluminium salt-based nanoparticles that may ultimately be used as adjuvant in human vaccines.

Promotores: Wim Jiskoot; Ferry Ossendorp

Promotor: Prof.dr. M. Danhof

This thesis describes the design, characterization and application of dextran based crosslinked network.

The work described in this thesis focuses on the development of linear or cyclized peptide probes against protein N-methyltransferases to characterize their specific binding behavior, providing further binding details for inhibitory activity study.

Promotor: Prof.dr. E. Bouwman, Co-Promotor: S. Bonnet

In dit proefschrift wordt de samenwerking tussen studenten en resultaten in online projectgestuurd hoger onderwijs in China onderzocht.

A new type of tripodal squaramide-based supramolecular hydrogels is developed and studied.

This thesis explores squaramide-based supramolecular polymers and hydrogels, enhancing their mechanical strength for 3D cell culture.

Promotor: T. Hankemeier, Co-promotor: T.H. Reijmers

Inspired by natural photosynthesis, perovskite-based photoelectrochemical (PEC) systems are being developed for artificial photosynthesis, aiming to enhance solar-to-hydrogen conversion for green energy.

Promotor: Prof.dr. J. Kuiper

The adenosine receptors are proteins that reside in the extracellular membranes of cells. Activation of adenosine receptors plays a role in many physiological and pathological processes, such as immune responses and cancers.

Promotores: Prof.dr. W.J.G.M. Peijnenburg & Dr. M.G. Vijver

Glycosidases are essential in fundamental biological processes and are responsible for the degradation of most (oligo)saccharides, glycolipids and glycoproteins.

Lysosomal glycosidases are acid hydrolases that fragment glycoconjugates in lysosomes.

Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel

Over the last decades, aviation safety has improved strongly. As a downside, airline pilots do not have as many opportunities to develop through experience the competencies that they need in critical situations.

This thesis focuses on the application of lipid-based nanomedicine in drug delivery, including small molecular antitumor drugs and biomacromolecules including mRNA, and evaluates their biological performance.

Meer informatie over dit onderzoeksproject vind je op de Engelstalige pagina.

Traditional drug discovery approaches have been hampered by (in vitro) cell-culture models that poorly represent the situation in the human body.

The chemical and structural heterogeneity of toxoid vaccines makes their analysis challenging. However, detailed insights on a molecular level can be obtained by mass spectrometry.

Antibiotic resistance, caused by widespread use of antibiotics, leads to bacterial infections that are difficult, if not impossible, to treat and is a major worldwide health concern.

Tin electrodeposition applications have rapidly evolved in the past 25 years.

Cell-based medicinal products (CBMPs), such as CAR T cells, are highly complex biopharmaceutical products offering various treatment options for serious illnesses such as cancer or autoimmune diseases.

Promotor: Prof.dr. B.R. Katzy

Promotor: Prof.dr. M. Danhof, Co-Promotor: J. de Jongh

Promotores: W. Jiskoot, F. Ossendorp

This doctoral thesis focuses on the development of polypept(o)ide-based nanostructures for therapeutic and diagnostic applications. The work combines controlled polymer synthesis, nanocarrier engineering, and biological evaluation to address current challenges in nanomedicine, with a particular focus…

Promotor: Jan van der Greef; Co-promotors: Mei Wang; Eduard van Wijk