Kinase inhibitors are key therapeutic agents, particularly in oncology, yet their clinical efficacy is often hampered by off-target effects and limited understanding of their cellular target profiles.

Promotors: W. Jiskoot; G.J. Witkamp, Co-promotor: M.R. Nejadnik

The skin is our natural barrier and lipids are a key part of this barrier. In the outer skin layer, the stratum corneum (SC), lipids form a densely organized structure dependent on the composition of these lipids.

Source: Structure , Volume 22, Issue 8, pp. 1120-1139 (2014)

Promotor: Prof.dr. B. van de Water

Cardiovascular disease is the number one cause of death worldwide. The most important risk factor for developing this disease is high cholesterol levels in the blood.

Epithelial-mesenchymal plasticity (EMP) and tumor cell migration play an important role in cancer progression, and an improved understanding of the mechanisms underlying these concepts is essential for developing new targeted approaches.

Viral hemorrhagic fever (VHF) is a group of acute diseases caused by highly infectious viruses including Ebola, Lassa, Dengue viruses. Its high mortality rate poses high risk to public health, however, studies on VHF have been hampered due to the non-availability of proper models and incomplete knowledge…

There is an urgent need for more physiologically relevant cell culture methods to guide compound selection in pre-clinical stages of the drug development pipeline.

Traditionally, vaccines are administered intramuscularly using conventional hypodermic needles, which cause pain and distress. Microneedles are very short needles (smaller than 1 mm) that are practically invisible to the naked eye.

Promotor: Prof.dr. A. P. IJzerman, Co-Promotor: L.H. Heitman

Childhood obesity is an increasing health issue. In the first part of this thesis comorbidities in children with obesity were studied, concerning the diagnostic process and dosing regimens.

Promotores: B. van de Water; P. Hogendoorn; J. Bovée Co-Promotor: E.H.J. Danen

Neurodegenerative diseases, including Parkinson’s disease (PD), are increasing in prevalence due to the aging population. Despite extensive study, these diseases are still not fully understood and the lack of personalised treatment options that can target the cause of the diseases, rather than the symptoms,…

Promotor: Prof.dr. M. Danhof

In summary, the collective results described in this thesis show that nanoparticulate vaccines can be delivered intradermally by coated and hollow microneedles and evoke antigen-specific immune responses.

Promotor: Prof.dr. J. Kuiper

Cardiovascular disease is the leading cause of death worldwide.

The focus of the described research in this thesis is on the oxidative stress response (Nrf2 pathway). The aim of the research presented in this thesis is to obtain more information concerning microRNAs which are involved in the Nrf2 pathway, to determine and evaluate the application of microRNAs for…

Inflammatory skin diseases such as psoriasis and atopic dermatitis can severely impact both the physical and social aspect of a patient’s life. Aberrant inflammatory responses are thought to be a major causative factor in these disorders, but an abundance of different factors contribute to the development…

In this thesis, we have studied the potential of the zebrafish larval model in studying the ECS, as a complementary model to the existing rodent models.

This thesis focuses on using liposomes in two different treatment strategies; vaccination (or immunotherapy) and delivery of a small molecule, and in two different disease models; cancer and atherosclerosis.

Promotor: J. A. Bouwstra

Pancreatic ductal adenocarcinoma (PDAC) is among the cancers with the highest mortality rate, despite a relatively low incidence.

Breast cancer (BC) is a clinically and biologically heterogeneous disease.

Bij onderzoek naar geneesmiddelen zou meer gebruik moeten worden gemaakt van data science. Dat zegt Gerard van Westen, postdoctoraal onderzoeker bij het Leiden Academic Centre for Drug Research (LACDR). ‘Als je betere medicijnen wilt hebben, moet je starten bij data.’

Binnen de Faculteit der Wiskunde en Natuurwetenschappen zijn 8 instituten:

Acht op de tien experimentele geneesmiddelen overleven de testfase op patiënten niet. Piet Hein van der Graaf, hoogleraar Systeemfarmacologie, wil die verspilling van tijd en geld voorkomen met nieuwe meet- en rekenmethoden.

Onderzoek in het BioTherapeutics onderzoeksprogramma is gericht op een soepelere en snellere transitie van preklinisch onderzoek naar geneesmiddelen en op het afleveren van medicijnen in het menselijk lichaam met behulp van kleine moleculen en ‘biologics’.

Exploring metabolomics-based biomarkers for diagnosis and treatment response monitoring of community-acquired pneumonia

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This thesis describes our search to identify and understand possible regulatory mechanisms of integrin α6β4 in cell-matrix adhesion and intracelular signaling.

In vitro disease models play a fundamental role in drug research. They enable the systemic identification and validation of disease mechanisms and the discovery of possible drug targets. However, more than often, drug candidates that are identified in vitro fail in the later clinical stages of drug…

uman skin equivalents (HSEs) are in vitro 3D-skin models that mimic many aspects of the native human skin (NHS) and can be a valuable tool.

Student Molecular Science and Technologie Madouc Bergers maakte voor haar bachelorscriptie zelf een molecuul dat de afbraak van suikers kan remmen. Hoewel het de meeste studenten niet eens lukt om één bouwsteen te maken, maakte Madouc er drie. Mede hierdoor is ze genomineerd voor de Science Young Talent…

Neptunus is de achtste planeet vanaf de zon en staat op een afstand van 4,5 miljard kilometer. Hij staat zo ver af van de zon dat het wel 165 jaar duurt voordat Neptunus een rondje om de zon heeft gedraaid. Een dag op Neptunus is korter dan op aarde: in maar 16 uur draait Neptunus een rondje om zijn…

Tuberculosis (TB) is associated with 1.5 million deaths annually. There is a need exists to optimize both current as well as novel antibiotic combination treatment strategies to improve the effectiveness and safety of treatments against TB.

This thesis represents a comprehensive investigation into the control of cancer stemness and metastatic initiation using a combination of advanced zebrafish xenograft models and in vitro assays.

The aim of this thesis was to develop aluminium salt-based nanoparticles that may ultimately be used as adjuvant in human vaccines.

This thesis focuses on the development of sample-preparation methods for small amounts of samples and applying the developed methods to muscle tissues to investigate the mechanisms involved in sarcopenia.

The idea of citizens and public professionals collaborating to provide public services has widely spread in several countries around the globe. Co-production is often presented as the go-to solution for challenges like a legitimacy crisis of both the government and the market, and financial concerns.…

Als gevolg van de grote technologische vooruitgang in de gezondheidszorg worden in toenemende mate gegevens verzameld tijdens de uitvoering van klinische onderzoeken.

The overarching clinical aim of this thesis was to improve pharmacological pain management by characterizing the pharmacodynamics of analgesics. This promotion has been awarded the predicate cum laude.

Microglia support the central and peripheral nervous system by forming the first line of defense against hazardous events. Microglia are increasingly acknowledged as critical players in neurodegenerative diseases, highlighting them as potential therapeutic targets.

Promotor: A.P. IJzerman

Promotor: C.A.M.J.J. van den Hondel, Co-promotor: Sandra de Weert

Promotor: H.S. Overkleeft, J. Neefjes, Co-promotor: M. van der Stelt

De keten die patiënten voorziet van nieuwe medicijnen, bestaat uit diverse complexe schakels: van fundamentele wetenschap, tot klinische tests, tot productie. Deze hele keten is aanwezig in Leiden. De Universiteit Leiden, het Leids Universitair Medisch Centrum (LUMC) en de bedrijven op het Leiden Bio…

Bij kanker groeien lichaamscellen ongecontroleerd. Het gedetailleerd in kaart brengen van hoe dit precies gebeurt maakt het mogelijk om efficiënte therapieën te ontwikkelen. Onderzoekers van het Leids Universitair Medisch Centrum (LUMC) en de Universiteit Leiden werken samen aan het vermeerderen van…