The research aims to explore the evolutionary adaptability of enzymes and the impact of temperature on protein evolution pathways, using M. tuberculosis β-lactamase BlaC as the object of study. Enzymes inherently embody a delicate balance between activity and stability, and the acquisition of new enzymatic…

Due to the modern techniques of combinatorial chemistry and high-throughput screening, data on the biological activity of many millions of compounds is known. However, it is still very difficult to transfer this data into knowledge: if we know that compounds A and B bind to a certain protein with high…

Dr. at the Department of organic chemistry, Wageningen University - the Nederlands

The studies presented in the work show the potential of the integrative use of biophysical data in defining the structural basis of protein interactions.

Enzymes are innately sensitive to changes in the amino acid sequence, which largely constrains their evolutionary potential, i.e., evolvability. This evolutionary burden can be alleviated in the presence of stabilizing mutations, which increase the buffering capacity of enzymes to tolerate mutations…

A key step in the synthesis of oligosaccharides and glycoconjugates is the formation of the glycosidic bond.

This dissertation explores new chemical strategies for the synthesis of self-adjuvanting peptide conjugates and immunomodulatory ligands aimed at targeted activation of the immune system.

Promotor: M. Ubbink, Co-promotor: G. Siegal

Glycoside hydrolases (glycosidases/GHs) are widely abundant enzymes in all kingdoms of life and are important biocatalysts that catalyze the hydrolysis of glycosidic linkages in oligo/polysaccharides, glycoproteins and glycolipids with tremendous efficiency

In this thesis unconventional tools based on fluidic interfaces were developed to study the surface and interfacial chemistry of graphene, to characterize the intrinsic properties of graphene, to disentangle the effects of substrate and of the environmental factors, and to improve handling protocols…

This thesis focuses on developing wafer-scale two-dimensional (2D) materials by combining synthesis, transfer, characterization, and device integration of graphene, hexagonal boron nitride (h-BN), and BN-doped amorphous carbon.

Neurodegenerative diseases pose a large medical and societal challenge. The etiology of these diseases is still poorly understood, which makes drug discovery for these diseases difficult.

Lipid nanoparticles (LNPs) have enabled the success of mRNA vaccines but remain limited in broader therapeutic use by challenges in delivery efficiency, targeting, and mechanistic understanding.

Bioarchaeology covers the study of all biological remains from archaeological sites.

It’s been a turbulent, bizarre and extraordinary year, 2020. Coronavirus turned the lives of everyone at our University upside down. Out teaching, research and all the events that are held in a year: nothing was the same as before. That this affected all of us is clear from the highlights and many reactions…

This thesis describes the evaluation of novel natural products with antibacterial activity enabled by total chemical synthesis.

Division contact information & address

The drug BIA 10-2474 inhibits fatty acid amide hydrolase (FAAH), a lipase that degrades a specific endocannabinoid. On the basis of this activity, BIA 10-2474 was being developed as a potential treatment for anxiety and pain. In a phase 1 trial of the drug, one subject died, and four others suffered…

A high-end label: Cyclophellitol aziridine-type activity-based probes allow for ultra-sensitive visualization of mammalian β-glucosidases (GBA1, GBA2, GBA3, and LPH) as well as several non-mammalian β-glucosidases (see picture). These probes offer new ways to study β-exoglucosidases, and configurational…

Three at the same time: a ligation strategy combining tetrazine–norbornene cycloaddition, Staudinger–Bertozzi ligation, and copper(I)-catalyzed click reaction was used to label the three catalytic activities of the proteasome simultaneously in a single experiment. The orthogonality of the three ligation…

Nowadays children grow up with social media. This may influence the development of brain regions involved in social interaction. In their review article in Nature Communications, Crone and Konijn illustrate how neuroscience can contribute to a better understanding of how media and peers influence adolescents'…

Africa is online. Leiden Africa expert Mirjam de Bruijn is fascinated by the fast development of mobile telephony and social media in Africa. She maintains a website on the topic, focusing on isolated, marginalised and conflict-ridden areas in Middle Africa.

On 4 and 5 September, Leiden Law School hosted a special conference that brought together around sixty participants from the Netherlands and abroad to discuss the future of economic, social, and cultural rights – rights that touch upon everyone’s daily life: housing, education, healthcare, food, and…

Siemens recently took over the Leiden software company Culgi, founded by professor and inventor J.G.E.M. (Hans) Fraaije. We spoke to him about the algorithm that made him successful, the role of a university in our society and his ambitions at Siemens. ‘I was looking for Siemens, and they were looking…

In June the Final Festival took place, the closing event of the 'Social Science Lab'. New participants of Honours College Science and Society presented their solution for a current social problem.

Compare human social behaviour at a country level and you will find differences. Japan has the highest score whereas the Netherlands is just above average. This is what psychologist Niels van Doesem discovered in research with an international team of 64 colleagues in 31 industrialised countries. Their…

A 5 million euros grant from the Dutch Research Agenda allows Anouk de Koning and co-applicants Femke Kaulingfreks and Maartje van der Woude to study social interventions in eight Dutch cities in an innovative and interdisciplinary way.

What interests and motives shape how people cooperate, clash and place their trust in one another? For more than 36 years, Van Dijk explored these questions using experimental games in the laboratory. 'Games strip complex issues back to their core.'

During the event 'Fout?' by De Jonge Akademie, Ellen de Bruijn held a lecture about the social context of making mistakes and the psychological elements of learning from it.

Could policies aimed at preventing radicalisation undermine the very trust and social cohesion they aim to strengthen?

Monday 26 September, 2016 saw the first confrontation between Hillary Clinton and Donald Trump. Expectations were high – not only about the content of the debate, but also about how the two presidential candidates would behave, and how this might influence their campaigns. We asked three researchers…

Organised jointly by the Europa Institute, Leiden University and the Lisbon Centre for Research in Public Law, University of Lisbon on 31 May 2021.

Double Bind. Public service professionals coping with contrasting demands. How do public service professionals align their PSM with contrasting demands set by the organizational and social contexts in which they work?

The main research theme in the group of Dennis Hetterscheid is to understand and mimic bioinorganic multi-electron processes that are relevant to our future energy infrastructure. Reduction of protons generates hydrogen that can be used as a chemical fuel. Alternatively to gaseous hydrogen, the reduction…

The Leiden Institute of Chemistry (LIC) offers a unique tailor-made programme in Life Science and Technology. You can customize the programme to your own interests and ambitions with the help of your own personal mentor. Your study will focus on either a biomedical, molecular or biophysical approach…

The thesis describes the development, synthesis and biological evaluation of several proteasome inhibitor designs. Mainly, this work focusses on designing proteasome inhibitors that selectively inhibit the immunoproteasome, while leaving the constitutive proteasome mostly, if not completely, untouch…

There are eight institutes at the Faculty of Mathematics and Natural Sciences:

This thesis describes the design and synthesis of novel small molecules based on sulfur-containing heterocycles for the inhibition of glycosidases and glycosyltransferases related to Fabry and Pompe disease.

The cannabinoid receptor type 2 (CB2R) is associated with several inflammatory diseases with an unmet medical need (e.g. Alzheimers, multiple sclerosis, reumatoid arthritis). Development of new chemical biology strategies to study this protein is essential to aid future development of drugs for these…

A deceptively simple, complicated paper

The aim of the research described in this thesis was to use structure-based design to optimise multiple properties of aryl sulfoxide monoacylglycerol lipase inhibitors as anti-inflammatory analgesics.

Thesis focused on uncovering the underlying mechanisms of the lysosomal storage disorders Gaucher's disease using chemical tools, biochemical assays and lipidomics.

Inaugural lecture