This thesis investigates durational aspects of the Italian mobile diphthongs with respect to other rising diphthongs and monophthongs and it shows to what extent the monophthong/diphthong alternation still occurs in contemporary Italian.

Promotores: T.H.W. Bäck, A. Plaat, Co-promotor: F.J. Verbeek

To what extent are there differences between countries in and outside the European Union and the Schengen area in the level of crimmigration, the merger between migration control and crime control, and to what extent can these differences be explained by the way in which state and non-state actors in…

Streptomyces are multicellular, Gram-positive bacteria in the phylum of actinobacteria which produce a high amount of bioactive natural products of which the expression is tightly coordinated with the life cycle.

The loss of T-cell homeostasis and tolerance towards self-antigens is the underlying cause of autoimmune and inflammatory diseases like atherosclerosis, rheumatoid arthritis or type I diabetes. Traditionally, the treatment of these diseases consisted on systemic immune suppression, which can entail…

One of the major limitations in culturing complex tissues or organs is the lack of vascularization in the cultured tissue. Development of a functional capillary bed could overcome this problem.

In our research protein translocation from Agrobacterium into yeast and plant cells is studied to obtain fundamental insights in the translocation process

An analysis of researchers' global mobility reveals that limiting the circulation of scholars will damage the scientific system.

Promotor: Prof.dr. R. Verpoorte, Co-Promotores: N.R. Mustafa, A.E. Schulte

Promotor: C.A.J. Knibbe, Co-promotor: J.J. Boelens, R.G.M. Bredius

The deeper understanding of an organism's pathology is important for developing treatments. Over centuries of systematic research, clinical researchers have demonstrated that the more information they acquire about the cellular properties and their organisation in the tissue, the better they can understand…

In this thesis, the focus lies on studying glucocorticoid receptor dynamics in living cells with the aim of understanding how this transcription factor finds its DNA target sites to regulate transcription.

Promotor: A.H. Meijer, Co-promotor: B.E. Snaar-Jagalska

To what extent are there differences between countries in and outside the European Union and the Schengen area in the level of crimmigration, the merger between migration control and crime control, and to what extent can these differences be explained by the way in which state and non-state actors in…

In the 4 October, 2017 issue of Nature appeared the comment entitled ‘Scientists have most impact when they're free to move’, an analysis measuring the global movements of researchers and reflecting on the potential adverse consequences that isolationist policies may have on the advance of science.

Eduard Fosch-Villaronga, Associate Professor at eLaw, contributed to 'Cyber–Physical–Human Systems', a book exploring the latest developments in interactions between cyber–physical systems and humans.

Since the emergence of humankind people have maintained social contacts and traveled widely, establishing interaction networks in which goods are traded and ideas are transmitted, increasingly on a global scale.

The lipid matrix of the skin's stratum corneum plays a key role in the barrier function, which protects the body from desiccation. The lipids that make up this matrix consist of ceramides, cholesterol, and free fatty acids, and can form two coexisting crystalline lamellar phases: the long periodicity…

Light-Harvesting Complex II (LHCII) is responsible for light absorption and excitation energy transfer in plants and photosynthetic algae, while in high light it undergoes conformational changes by which it quenches excitations to prevent photodamage.

This thesis describes the antimicrobial discovery strategy developed in our group, the den Hertog Group at the Hubrecht Institute. It includes a cultivation-based screening approach for novel antimicrobial agents from the source of fungi, and a bacterial time-lapse imaging approach for antimicrobial…

This thesis focused on the exploration of monolayer graphene as a membrane in direct methanol fuel cell (DMFC) applications, with improvements achieved through plasma exposure or damage- free chemical functionalization.

Dendritic cells (DC) play a prominent role in the priming of CD8(+) T cells. Vaccination is a promising treatment to boost tumor-specific CD8(+) T cells which is crucially dependent on adequate delivery of the vaccine to DC.

The aim of this work was to study the applicability of antigen-coated pH-sensitive microneedle arrays for effective vaccination strategies. Therefore, a model antigen (ovalbumin) was coated onto pH-sensitive (pyridine-modified) microneedle arrays to test pH-triggered antigen release by applying the…

The emergence of complex diseases resulting from abnormal cell-cell signaling and the spread of infectious diseases caused by pathogens are significant threats to humanity. Unraveling the dynamic mechanisms underlying cell-cell signaling and infectious disease spreading is crucial for effective disease…

In this book chapter, Andrea Malaya M. Ragragio and Myfel D. Paluga unpack the indigenous category

Promotors: Prof.dr. G.R. de Snoo, Prof.dr. G.A. Persoon, Prof.dr.ir. H.H. de Iongh

Poly(lactic-co-glycolic acid) (PLGA) particles have been extensively studied as biodegradable delivery system to improve the potency and safety of protein-based vaccines. In this study we analyzed how the size of PLGA particles, and hence their ability to be engulfed by dendritic cells (DC), affects…

This thesis describes the development of a variety of mannosylated conjugates. Antigen presenting cells bear mannoside recognizing receptors that actively transport antigen into the cell. This thesis exploits this feature for the development of improved vaccines.

A very large part of intravenously administered nanoparticles are cleared through the liver. Within the liver, most nanoparticles are thought to be sequestered by macrophages (Kupffer cells). To achieve effective cell-specific targeting of drugs and non-viral gene delivery vectors, improved mechanistic…

PhD defence

Cell-based medicinal products (CBMPs) show great promise when it comes to the treatment of genetic disorders. However, there is limited knowledge about CBMP stability during their production, storage, and administration to patients. The main focus of my research is the characterization of CBMPs using…

PhD defence

PhD defence

For effective cancer immunotherapy by vaccination, co-delivery of tumour antigens and adjuvants to dendritic cells and subsequent activation of antigen-specific cytotoxic T cells (CTLs) is crucial. In this study, a synthetic long peptide (SLP) harbouring the model CTL epitope SIINFEKL was encapsulated…

How does an embryonic stem cell decide if it becomes a heart cell or a kidney cell? That’s the question computational biologist Maria Mircea studied for her PhD research. She looked at the inside of individual cells to analyse how they change. This is what she discovered.

Single-Molecule Microscopy (SMM) techniques constitute a group of powerful imaging tools that enable researchers to study the dynamic behavior of individual molecules.

Thread-like bacteria make cells that no longer have a cell wall under the influence of osmotic stress. A remarkable discovery, since the cell wall serves as a protection barrier for bacteria. It could also help to explain how pathogenic bacteria can hide in our body from our immune system. A team of…

On 12 November, Clare Fenwick defended her doctoral thesis, The Political Economy of Immigration and Welfare State Reform: A Collection of Comparative Political and Economic Essays on Human Mobility and Social Protection. The PhD research was supervised by Olaf van Vliet and Kees Goudswaard.

The LUMC has become the first institution in the Netherlands to treat a patient with an autoimmune disease using CAR T-cell therapy.

The Leiden University Medical Center (LUMC), the Danstem Institute from the University of Copenhagen and the Murdoch Children’s Research Institute in Melbourne have received 300m euros from the Novo Nordisk foundation. The aim of this new international consortium is to bring stem-cell based therapies…

Kidney failure is a serious issue because kidneys cannot regenerate themselves after injury. A possible solution consists of artificially growing healthy kidney tissue. To achieve that, scientists first need to understand kidney development during the earliest stages, in the fetus. Leiden researchers…