Promotor: Prof.dr. R. Verpoorte, Co-Promotores: N.R. Mustafa, A.E. Schulte

In het schoolvak scheikunde is de concept-context benadering ingevoerd. Hans Vogelzang onderzocht of de Scrum-methodologie kan helpen bij het vormgeven van lessen met deze benadering.

This dissertation contains four works during my PhD. Different biomaterials have been designed based on coiled-coil peptides. These biomaterials have a range of applications, inclusing drug delivery, cell sorting to cell-cell fusion.

This thesis describes the antimicrobial discovery strategy developed in our group, the den Hertog Group at the Hubrecht Institute. It includes a cultivation-based screening approach for novel antimicrobial agents from the source of fungi, and a bacterial time-lapse imaging approach for antimicrobial…

The deeper understanding of an organism's pathology is important for developing treatments. Over centuries of systematic research, clinical researchers have demonstrated that the more information they acquire about the cellular properties and their organisation in the tissue, the better they can understand…

The way organisms develop from the initial single-cellular state to a complex final assembly like the human body, and how the final body is maintained throughout life, is one of the greatest mysteries and it’s understanding one of the biggest scientific challenges.

The emergence of complex diseases resulting from abnormal cell-cell signaling and the spread of infectious diseases caused by pathogens are significant threats to humanity. Unraveling the dynamic mechanisms underlying cell-cell signaling and infectious disease spreading is crucial for effective disease…

Promotor: A.H. Meijer, Co-promotor: B.E. Snaar-Jagalska

The overall theme of this thesis is to complement the electrochemical data acquired in fuel cell research with in situ X-ray photoelectron spectroscopy (XPS) and soft X-ray absorption spectroscopy (XAS).

This thesis is largely an experimental study on the formation of solid-state simple and complex organic molecules in the H2O-rich and CO-rich ice phases of dense interstellar clouds and dark cores.

Multiple stars, that is two or more stars composing a gravitationally bound system, are common in the universe.

Promotor: J. Schaye

This thesis examines the link between simple molecules and the underlying structure and chemistry within protoplanetary disks - the birthplaces of planets.

In order to be able to develop effective medicine and treatments to prevent or cure autoimmune diseases or cancer we need to understand the mechanisms how they arise and what drives their course.Unravelling the fundamental molecular mechanisms influencing the onset and course of diseases such as allergies,…

In this thesis, bioorthogonal chemistry is combined with correlative light-electron microscopy to selectively label and study pathogenic intracellular bacteria within the host immune cell.

In photoactivated chemotherapy (PACT), a biologically active compound is caged by a light-cleavable protecting group.

Promotor: G.A. van der Marel, Co-promotor: J.D.C. Codée

Advanced mass spectrometry of glycosphingolipids takes the central stage in this thesis. Investigations focus on characterization of glycosphingolipid metabolism in health and disease with emphasis to the detection and accurate quantitation of known and so far unknown glycosphingolipids and closely…

With the rise of multi-drug resistant bacterial pathogens, the possibility of a post-antibiotic world is quickly becoming reality.

To address the fundamental questions of how life on Earth emerged and how common life may be in the Universe, it is crucial to know the chemical composition of the planet-forming material.

Promotor: J.W.M. Frenken Co-Promotores: I.M.N. Groot; L.B.F. Juurlink

Decentralized plug and play systems for energy production are the future picture of our society. Artificial photosynthetic systems are used for this purpose.

The main aim of this thesis was to unravel the signaling and regulatory networks that drive tumor cell migration during breast cancer metastasis.

Onderwijsminister Jet Bussemaker kreeg op de Dag van de Excellentie op 20 juni een exemplaar van ‘Chemistry in Conflict’, een educatief boek over de rol van chemie in oorlogsvoering. Ludo Juurlink, directeur van het Junior Science Lab, overhandigde het boek.

Viral hemorrhagic fever (VHF) is a group of acute diseases caused by highly infectious viruses including Ebola, Lassa, Dengue viruses. Its high mortality rate poses high risk to public health, however, studies on VHF have been hampered due to the non-availability of proper models and incomplete knowledge…

In eight chapters, the thesis of Rafaël Vos covers the effect of temperature and pressure on the electrochemical CO2 reduction.

Carbohydrates are present on the surface of bacteria making them suitable antigen candidates for vaccine development. This thesis deals with the synthesis of two carbohydrate-based components; the capsular polisaccharide of S. aureus type 5 and teichoic acids from staphilococci and enterococci speci…

In this thesis, the focus lies on studying glucocorticoid receptor dynamics in living cells with the aim of understanding how this transcription factor finds its DNA target sites to regulate transcription.

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This thesis advance our understanding and the application of molecular catalysts relevant to the reduction of oxygen as well as reduction of nitrite.

Details kunnen zien van een zieke of gezonde cel, dat is mogelijk met de supermicroscoop van het consortium Cyttron. Niet alleen de microscoop is vernieuwend in zijn soort: ook de vorm waarop de partijen samenwerken past in de huidige tijd. “Toegang tot elkaars techniek, daar draait het om in ons samenwerkingsverband”,…

Met de nodige maatregelen starten onderzoekers hun werkzaamheden in verschillende laboratoria weer deels op. Het Leidse Cell Observatory is één van de plekken waar wetenschappers sinds 11 mei hun labwerk hervatten.

Reducing greenhouse gas emissions is one of the most pressing challenges that humanity faces in the coming decades.

The main goal of the research described in this thesis was the development of new photoactivated chemotherapy (PACT) ruthenium(II) complexes bearing a non-toxic photolabile ligand.

Promotor: Prof.dr. T. Schmidt

Cancer is considered the silent pandemic of the 21st century and the second leading cause of death worldwide.

Promotor: A.P.IJzerman Co-promotor: A. Bender

The cellular metabolism of immune cells constitutes an important part of their proper function and activation. Using T cells as an example, this phenomenon is highlighted by the metabolic reprogramming that occurs in these cells upon activation.

Synthesis and application of chemical biology tools to study immunomodulatory signaling lipids.

A dense region of a gaseous and dusty cloud collapses to form a protostar surrounded by a disk and an envelope. This thesis uses both observations and models to study physical and chemical conditions of these protostellar systems which are likely where planets start to form.

Despite the fact that gold nanoparticles (GNPs) are one of the most studied nanoparticles, there is still a necessity for new approaches allowing for effective protective coating to enable wider use of GNPs in biomedical applications.

The research described in this thesis focused on the preparation of S/N functionalized carbene ligands and their transition metal complexes, and the exploration of their application as electrocatalysts for proton reduction.

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Promotor: Prof.dr. G.P. van Wezel

This thesis describes the use of bioorthogonal proteins in immunological settings. It provides an introduction towards the field of protein modification, which was used throughout the thesis for the expression of proteins containing unnatural amino acids.

This thesis has described novel synthetic methods to produce a variety of (glyco)peptides and their application in the study of various immunological processes.

The inverse electron demand Diels-Alder (IEDDA) pyridazine elimination emerged in 2013 as a new bioorthogonal reaction and constitutes a prime example of what is now known as dissociative bioorthogonal chemistry. The research described in this Thesis aims to develop synthetic strategies which enable…

Carbohydrates, or sugars, are the most diverse and most abundant biomolecules known. However, the isolation of carbohydrate samples in sufficient amounts and purity is often impractical or even impossible, so the chemical synthesis of glycosides becomes relevant. The glycosylation reaction, in which…