The spindle-assembly checkpoint (SAC) is a safety mechanism which secures accurate chromosome segregation during mitosis.

This PhD-thesis presents a study on micron-sized particles, so-called colloids. By controlling the chemical and physical properties of these particles, such as the interparticle interaction and the particles’ shape, colloids can act as building blocks that self-assembly into larger structures.

Advanced sensing techniques require graphene with high quality and well-controlled surface chemistry.

In this dissertation iron-based homogeneous catalysts were synthesized, characterized and investigated for water oxidation activity.

Promotor: J.G.E.M. Fraaije, Co-Promotor: A. Kros

The lipids in the uppermost layer of the skin, the stratum corneum (SC), play an important role in the skin barrier function. The three main SC lipid classes are ceramides (CER), cholesterol and free fatty acids.

Fungal food spoilage often starts with a contamination with spores. Experimental data strongly indicate the existence of subpopulations of spores with different levels of resistance to preservation methods.

Stars and planets form within cold, dense clouds of gas and dust drifting through interstellar space. Although dust makes up only a small fraction of this material, it plays a key role in shaping the chemical evolution of these environments.

The chemical and structural heterogeneity of toxoid vaccines makes their analysis challenging. However, detailed insights on a molecular level can be obtained by mass spectrometry.

A century ago, it was unclear whether the stars in the sky were clustered in groups, or widely spread in the universe.

The work described in this dissertation contributes to a better mechanistic understanding of nanoparticles in vivo. To achieve that goal, we used the zebrafish as a highly predictive pre-screening model of nanoparticles.

This thesis describes the design and synthesis of fragments of various cell wall carbohydrates of the Streptococcus species, including the branched Group B-specific antigen (GBC) of Group B Streptococcus, glycerol phosphate (GroP) modified group A carbohydrate (GAC), and the O-acetylated type 1 capsular…

As leading cause of death worldwide, cancer is responsible for nearly 10 million deaths in 2020 according to World Health Organization (WHO). Cisplatin and its derivatives are commonly used chemotherapy agents for current cancer treatment in the clinics.

Promotores: T. Hankemeier; R. Berger, Co-promotor: R.J. Vreeken

This thesis describes the use of bioorthogonal proteins in immunological settings. It provides an introduction towards the field of protein modification, which was used throughout the thesis for the expression of proteins containing unnatural amino acids.

This dissertation, Layered Loyalties: The Natuurkundige Commissie in the Netherlands Indies (1820-1850), studies the Natuurkundige Commissie.

In my research, I used a comparative wood anatomical approach to address wood evolution of the (largely) tropical flowering plant families Apocynaceae and Sapotaceae.

Producing green energy has become the main goal in our society in the search of reducing or eliminating the carbon emission from fossil fuels.

The main subject of this thesis is the design and synthesis of paramagnetic molecules for protein studies with NMR and EPR spectroscopy.

Monoacylglycerol lipase (MAGL) is the principal enzyme responsible for hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-AG). MAGL inhibition provides several potential therapeutic opportunities, including anti-nociceptive, anti-inflammatory and anti-cancer activity.

Gaucher disease is a rare metabolic disorder, in which a mutation of the enzyme glucocerebrosidase (GBA1) causes buildup of its substrate glucosylceramide and the deacylated counterpart glucosylsphingosine in lysosomes.

This thesis describes how to scale down concepts of macroscopic mechanical metamaterials to the thermal scale using a system of flexibly-linked colloids.

In this thesis the links between allergy and intestinal health, the gut microbiome (GM), and external factors were studied by exploring the metabolome in longitudinal clinical studies and in vitro models.

Large parts of the research described in this Thesis aims at the development of oligopeptide-masked toxins and their in situ immunoproteasome-mediated activation.

Koreastudies in Leiden, Korean studies, Japanese studies, Chinese Studies, Asian Studies, South and Southeast Asian Studies, International Studies, Film en Literatuurwetenschap

In this dissertation (titled: Exploring the Synergies between Transfer in Reinforcement Learning and Procedural Content Generation) we explore how the two research fields named in the title, namely Transfer in Reinforcement Learning (TRL) and Procedural Content Generation (PCG) can synergize togethe…

Promotores: Prof.dr. G.A. Persoon & Prof. dr. H.H. de Iongh

Promotor: Prof.dr. M.A.G.J. Orrit

From surface science towards industrial practice using high-pressure scanning tunneling microscopy.

In order to gain a better insight into the basis of the rapid jasmonic acid (JA)-mediated stress response, an integrated approach using a targeted high-resolution mass spectrometry-based (HRMS) platform, was conducted to monitor the metabolism of JA using the model system of cell suspension cultures…

In this thesis, we used genetically engineered mouse models to identify genes and pathways that are involved in ILC formation and in the development of resistance to FGFR-targeted therapy.

In his dissertation Michiel Hooykaas outlines the results of six empirical research projects focused at biodiversity awareness in the Netherlands, specifically people’s knowledge about animals.

Promotors: Prof.dr. G.R. de Snoo, Prof.dr. G.A. Persoon, Prof.dr.ir. H.H. de Iongh

Promotor: Prof.dr. G.R. de Snoo

Promotor: G.P. van Wezel, Co-promotor: Y.H. Choi

Promotores: Prof.dr. C.A.M.J.J. van den Hondel, Prof.dr. V. Meyer (Technische Universität Berlin)

Breeding for resistance becomes more and more important because we want to reduce the use of pesticides. A fast and cheap alternative can be to make use of morphological or chemical markers.

In this thesis, mathematical modeling and simulation was applied as a tool to inform quantitative decision making in oncology drug discovery and development.

The upscaling of Life Cycle Assessment (LCA) data is recognized as one of the main challenges in the ex-ante LCA of emerging technologies. This challenge arises since industrial-scale system specifications for these technologies are unknown and industrial data is lacking.

This thesis describes the structural and biochemical characterization of the β-lactamase BlaC from Mycobacterium tuberculosis (Mtb), and the Alr and YlmE proteins from Streptomyces coelicolor A3(2).Mtb is the main cause of tuberculosis.

Actinobacteria are Gram-positive bacteria that have a complex multicellular life cycle and are well known for their ability to produce a wide range of bioactive natural products (NPs).

Promotores: Prof.dr. J. A. Bouwstra, Prof.dr. W. Jiskoot

Promotor: Prof.dr. M. de Jong, Co-Promotor: A.J. Heijboer

Promotor: T. Hankemeier, Co-promotor: R.J. Vreeken

The thesis deals with the study of giant radio galaxies (GRGs), which are radio galaxies of megaparsec sizes.

This thesis investigates the structural evolution and assembly of galaxies since the first few billions years after the big bang.