The Skin Barrier and Vaccination group is led by Prof. Joke Bouwstra. One of the key functions of the skin is its barrier function, which is located in the uppermost layer of the skin, the stratum corneum. The stratum corneum consists of enucleated dead cells (corneocytes) embedded in a lipid matrix.…

The currently available in vitro generated human skin equivalents resemble the human skin in many aspects. However, some essential barrier characteristics do not fully mimic the native barrier. Consequently, the human skin equivalents cannot be used for screening of drugs for skin penetration.

The skin is our natural barrier and lipids are a key part of this barrier. In the outer skin layer, the stratum corneum (SC), lipids form a densely organized structure dependent on the composition of these lipids.

Focus in skin research

Promotor: Prof.dr. J.A. Bouwstra, Co-promotor: Dr. A. El Ghalbzouri

Promotor: J.A. Bouwstra Co-promotor: A. El Ghalbzouri

The stratum corneum is the outermost skin layer and consists of dead cells embedded in a lipid matrix.

Human skin equivalents (HSEs) can be a valuable tool to study aspects of human skin, including the skin barrier, or to perform chemical or toxicological screenings.

In the studies described in this study, we introduce a novel ex vivo human skin barrier repair model. To develop this, we removed the upper layer of the skin, the stratum corneum (SC) by a reproducible cyanoacrylate stripping technique.

The research described in this thesis shows that hypercholesterolemia, a well-established risk factor for atherosclerosis, can impact skin lipid pool and barrier function already at young age. In the field of atherosclerosis, we showed that the small peptide Lyp-1 can be used as a targeting molecule…

Source: International Journal of Cosmetic Science, Volume 37, Number 1, pp. 148-148 (2015) ISBN: 0142-5463

The lipids in the uppermost layer of the skin, the stratum corneum (SC), play an important role in the skin barrier function. The three main SC lipid classes are ceramides (CER), cholesterol and free fatty acids.

The barrier function of the skin is primarily provided by the stratum corneum (SC), the outermost layer of the skin. Skin barrier impairment is thought to be a primary factor in the pathogenesis of atopic eczema (AE).

Source: International Journal of Cosmetic Science, Volume 37, Number 1, pp. 158-158 (2015) ISBN: 0142-5463

When does radicalization not lead to terrorist attacks?

Atopic eczema is a skin disease with increasing incidence currently affecting 25% of the paediatric population. In atopic eczema the skin barrier function is reduced. This barrier is formed by the outermost layer of the skin called the stratum corneum (SC). The SC consists of terminally differentiated…

Promotor: J. A. Bouwstra

Barrier function is the natural role of the skin. The lipid matrix present in the outermost layer of the skin, the stratum corneum is important for this function.

The skin protects the body from unwanted influences from the environment as well as excessive water loss. The barrier function of the skin is located in the stratum corneum (SC).

The skin barrier function is provided by the stratum corneum (SC). The lipids in the SC are composed of three lipid classes: ceramides (CERs), cholesterol (CHOL) and free fatty acids (FFAs) which form two crystalline lamellar structures. In the present study, we investigate the effect of CER chain length…

It is the aim of this project to better understand how the physical properties of lipids influences the structure and overall barrier function of the skin.

The outermost layer of the skin, the stratum corneum (SC), is responsible for the skin barrier function, protecting the body from pathogens, chemicals and other unwanted substances from the external environment. The SC lipid matrix provides the only continuous pathway through the SC and is considered…

uman skin equivalents (HSEs) are in vitro 3D-skin models that mimic many aspects of the native human skin (NHS) and can be a valuable tool.

OBJECTIVES: To determine whether the lipid/protein ratio and the total dry SC mass per surface area are related with the skin barrier function of controls and AE patients.

An important feature of atopic eczema (AE) is a decreased skin barrier function. The stratum corneum (SC) lipids - comprised of ceramides (CERs), free fatty acids (FFAs) and cholesterol - fulfill a predominant role in the skin barrier function.

The effectiveness of the skin barrier underlies the outer layer of the skin: the stratum corneum (SC). However, in several skin diseases this barrier is impaired. In two inflammatory skin diseases, atopic eczema and Netherton syndrome, an increased level of monounsaturated fatty acids (MUFAs) has been…

Netherton Syndrome (NTS) is a rare genetic skin disease caused by mutations in the serine protease inhibitor Kazal-type 5 gene, which encodes the lympho-epithelial Kazal-type-related inhibitor. NTS patients have a profound impaired skin barrier function. Because SC lipids play a crucial role in the…

This dissertation takes an important step in understanding the phenomenon of non-take-up of social support and what it means for contemporary social policies.

This paper describes two synthetic lipid models designed to replace human stratum corneum (SC) in studies of the impact of volatile organic chemicals on the molecular organization of the skin barrier lipids.

What is the relation between the experience and the act of exclusion?

CT scanning of skeletal remains can tell us about how strong or weak a persons bones were and we can relate that back to diet, activity, and reproductive factors.

The consequences of social tendencies for the legislation and institutions in the fields of social security, the labor market policy and pension reform are examined in this multdisciplinary research program..

Why does social anxiety increase in adolescence and how does it grow out of control in some adolescents?

Towards a Biological Understanding of the Middle to Upper Palaeolithic Transition

Source: British Journal of Dermatology, Volume 170, Number 6, pp. E18-E19 (2014) ISBN:0007-0963

This project aims at studying the social media reception of African publications as covered by the most important “altmetric” sources.

Our mission: a positive impact. Our vision: community. By involving the world around us in our education and research we make a positive impact. We do this by applying Open Science.

Why do some children confront social challenges with confidence, whereas others experience difficulties? What is the role of child temperament and child emotional dispositions? What is the role of parenting? This project aims to shed light on the mechanisms explaining shyness and confidence in response…

Are there any differences in gaze behavior between high and low socially anxious individuals? What factors influence the relation between social anxiety and gaze behavior?

A neurobiological perspective on individual differences in social competence

The studies outlined in this volume explore how connectedness continues to change Africa and how Africa continues to shape the social life of connections.

The key subject of the Team Economic and Social History is Inequality (at local, national and global levels). We study this from an intersectional perspective: gender, class, ethnicity or race, religion, sexuality, age, ability/disability, citizenship and legal status. We study these categories of power…

Do you have to be able to say everything on Twitter and Facebook? Is Instagram morally obliged to remove photos from attacks? Should we allow the terrorist group to recruit new members on the internet?

This project investigates how people choose linguistic forms like questions or statements in everyday speech.

At the Young Academy Leiden, we care deeply about fostering social safety at our university. Increasing social safety is of particular importance to early career scholars as they often find themselves in a vulnerable position without a permanent contract, or with a dependence on superiors for progressing…

Helping people, groups and policymakers navigate the social transitions of our time.

At FGGA, we want our staff to feel safe in all respects. We therefore strive to create a work environment where we treat each other with respect, where we promote inclusion and where we speak up if we experience or witness unsafety.

Main challenges in skin vaccination are overcoming the stratum corneum (SC) barrier and targeting the antigen presenting cells (APC) in the epidermis and the dermis.

This COIn Grant 2025 awarded project allows Leiden University researchers and students to do research on the world’s largest corpus of digitized Ottoman language periodicals and books.

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