Electron microscopy has become an extremely important techniquein a wide variety of elds.

Molecular materials have been a subject of interest in fundamental research and applications for decades, and have been studied as bulk crystals, (thin) films and as individual molecules, due to the large variety in their properties. This dissertation explores pentacene crystals near the two-dimensional…

The aim of this facility is to accelerate biomedical research by providing access to advanced light and electron microscopy and by sharing expertise about sample preparation, imaging modalities, data storage and image analysis.

Promotor: Prof.dr. J.M. van Ruitenbeek, Prof.dr. R.M. Tromp

The Microscopy Unit houses, maintains and coordinates most of the microscopy equipment of the IBL. The available equipment ranges from conventional light and fluorescence microscopes, to confocal laser scanning and electron microscopes. In addition, infrastructure is available for histology, including…

This thesis presents insights from our study of various correlated electron systems with a scanning tunneling microscope (STM). In ordinary metals, electron-electron interactions exist, but get substantially screened due to the sheer number of electrons.

The endophytic microbiota is a community of various microorganisms that reside in plant tissue. These microbes are known to promote plant health and resilience, and their members do not simply exist side by side.

Bacteriophages, or phages for short, are the most abundant biological entity in nature. They shape bacterial communities and are a major driving force in bacterial evolution.

Bacterial chemosensory arrays are protein assemblies that are the key structural and functional component for motile bacteria to sense their internal or environmental chemical signals.

In this thesis, we develop a novel technique called Optical Near-field Electron Microscopy (ONEM), which aims to combine the advantages of both optical and electron microscopy: the high resolution of electron microscopy and the low sample damage of optical microscopy.

n this PhD thesis, the recombination of different atomic lattices in stacked 2D materials such as twisted bilayer graphene is studied. Using the different possibilities of Low-Energy Electron Microscopy (LEEM), the domain forming between the two atomic layers with small differences is studied.

Promotores: Prof.dr. H.P. Spaink & Prof.dr. P.C.W. Hogendoorn, Co-promotor: Dr. M.J.M. Schaaf

In condensed matter systems electron-electron interactions, negligible in everyday metals, can dramatically alter the electronic behavior of the system. Examples of such altered behavior include high-temperature superconductivity and modulation of the electron density.

Visualising structures of life

Promotor: Prof.dr. J.P. Abrahams, Prof.dr. M. van Heel

Cryogenic electron microscopy (cryo-EM) is a powerful technique used to visualize the inside of cells and to study specific protein complexes. Within this thesis, I describe the use of various cryo-EM techniques to gain insight into the structural changes of the human pathogen, Vibrio cholerae, as it…

The dissertation describes TEM experiments on heterogeneous catalysts.

This thesis described the development of novel scanning tunneling microscopy techniques to investigate strongly correlated electronic states in quantum matter.

This thesis describes a study from both a theoreticaL and an experimental point of view.

Promotor: T. Schmidt

Materials with strongly correlated electrons show some of the most mysterious and exotic phases of quantum matter, such as unconventional superconductivity, quantum criticality and strange metal phase.

The Netherlands Organisation for Scientific Research (NWO) has made a subsidy of 17 million euros available to further develop a Netherlands network for electron microscopy (NEMI). The network comprises five UMCs and eight universities, with Utrecht in the coordinating role. From Leiden, the Institute…

Single-Molecule Microscopy (SMM) techniques constitute a group of powerful imaging tools that enable researchers to study the dynamic behavior of individual molecules.

This is a yearly event where we move in one week from the physics of light through the composition of the microscope, fluorescence microscopy and end up with some image analysis All the lectures are backed by practicals to really put your obtained knowledge into practice

Ferritin is a spherical metalloprotein, capable of storing and releasing iron in a controllable way. It is composed of a protein shell of about 12 nm and within its cavity, iron is stored in a mineral form.

How do we experience electronic music? How does electronic music operate on perceptual, cognitive and affective levels? What are the common concepts activated in the listener’s mind when listening to electronic music? Why and how are these concepts activated?

In this thesis we investigated the ability of two-photon multifocal microscopy for single-molecule microscopy in live cells and organisms.

From The Cognitive Continuum of Electronic Music:

Promotor: T.H. Oosterkamp

What is the Electronics Department?

Chinese and Leiden scientists came together in Leiden to study the intricacies of modern visual techniques.

Leiden University, together with Utrecht University, the LUMC and 10 other Dutch universities and institutes, has been awarded a grant of more than €30 million in the NWO call Roadmap Large-scale Scientific Infrastructure (GWI).

In surface science there is great effort to move from studying simple, flat model surfaces in vacuum to investigating more complex model catalysts in gas environments (in situ). This thesis gives three examples of such studies using microscopy and spectroscopy.

In this thesis we use Josephson and noise scanning tunneling microscopy for the study of conventional, unconventional (iron-based) and disordered superconductors. On the one hand, Josephson scanning tunneling microscopy allows us to directly visualize the superfluid density with high spatial resolut…

We explore the possibilities to combine magnetic resonance techniques with atomic force microscopy together in a single microscope: the MRI-AFM, also called Magnetic Resonance Force Microscopy (MRFM).

Intrigued by the way cells autonomously regulate their fate, we strive to understand and visualize cellular processes as the basis of Cell Signaling.

Single-molecule spectroscopy has become a powerful method for using organic fluorescent molecules in numerous applications.

In this thesis, we describe the latest advances in SQUID-detected Magnetic Resonance Force Microscopy (MRFM).

In this thesis, we show how MRFM can usefully contribute to the field of condensed-matter.

International trade plays a critical role in global food security, with global consumption having highly localized environmental impacts.

In this thesis the combinatorial use of bioorthogonal labelling and Electron Microscopy (EM)-based imaging techniques is explored to enable observations of specific molecular targets in their ultrastructural context within the cell.

In this Ph.D. thesis we study the interaction of low energy electrons with thin materials, namely layered materials (graphene, hexagonal boron nitride, molybdenum disulfide) and organic films. At these low energies the quantum mechanical wavelength of the electron wave function is in the order of a…

Promotores: Prof.dr. C.U. Keller, Prof.dr. H.C. Gerritsen

Bacteria are everywhere. They are the most abundant organisms on earth and impact all aspects of our lives. They determine our health and shape our environment. Ariane Briegel, professor of Ultrastructural Biology, freezes bacteria super fast to gain a true-to-nature image of the internal and external…

ChEMBL is a large-scale drug discovery database containing bioactivity information primarily extracted from scientific literature.

Atoms and molecules are the basic units of matter. If we keep dividing a bar of gold or a glass of water into smaller parts, at the end we are left with a single gold atom or a water molecule.

Promotor: J.P. Abrahams, Co-promotor: T. Grüne

The main research question of this thesis was: Can nitrogen cycling on dairy farms on peat soil in the Dutch Green Heart area be improved by adapting management to the nitrogen supply from soil and by using other grassland species and crops without affecting milk production?