Cyclic peptides are investigated as a platform to induce different orientations between various ligands.

This work has described synthetic strategies towards well-defined structures resembling capsular polysaccharide (CPS) fragments, CPS mimics, teichoic acid (TA) fragments as well as a third-generation ring-closing tandem metathesis (RCM) linker to better exploit the potential of automated synthesis.

Promotor: G.A. van der Marel, Co-promotor: D.C. Codée

Promotores: H.S. Overkleeft, G.A. van der Marel, Co-Promotor: J.D.C. Codee

Schistosomiasis is an acute and chronic disease caused by blood dwelling parasitic trematodes of the genus Schistosoma, and it is classified as the second most socioeconomically devastating parasitic disease, second only to malaria.

An objective of this research was to achieve direct, well-defined and non-rate-limiting electron transfer between respiratory enzymes and the electrode surface by means of 'Q-wires'.

This thesis describes the synthesis and biological evaluation of TLR2/6, TLR4, TLR7/8 and TLR9 ligands, of which the activity can be conditionally controlled.

This Thesis describes the design, synthesis and evaluation as glycoprocessing enzyme inhibitors of focused libraries of iminosugars.

Het proefschrift omschrijft de chemische synthese van fenolische glycolipiden van verschillende mycobacteriën met het doel om deze te kunnen gebruiken voor immunologisch onderzoek.

Chapter 2 describes a post-synthetic methodology to introduce a fluorescent label in highly lipophilic, Pam3Cys based conjugates.

This thesis focuses on the design and synthesis of activity-based probes and inhibitors targeting enzymes involved in the degradation of specific plant glycans.

This thesis addresses the urgent challenge of antimicrobial resistance through the discovery and modification of natural product antibiotics.

Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel

Synthetic methodlogy is described, aiding in the synthetic preparation of putative inhibitors of retaining and inverting glycosidases and glycosyl transferases. All constructs are cyclophellitol-based cyclitols.

Sinds de uitbraak van de globale COVID-19 epidemie is het coronavirus dat daarvoor verantwoordelijk was, SARS-CoV-2, al veelvuldig onderzocht. Een van de enzymen belangrijk voor het virus, Mac1, kan bijvoorbeeld worden geremd door stofjes te ontwikkelen die dit enzym kunnen foppen.

This dissertation explores new chemical strategies for the synthesis of self-adjuvanting peptide conjugates and immunomodulatory ligands aimed at targeted activation of the immune system.

This thesis focuses on developing wafer-scale two-dimensional (2D) materials by combining synthesis, transfer, characterization, and device integration of graphene, hexagonal boron nitride (h-BN), and BN-doped amorphous carbon.

The thesis focuses on synthesis strategies in oligosaccharide campaigns, and the influence of protecting groups.

Sphingolipids are important membrane compounds with a variety of functions. In mammalian cells, different enzymes are involved in the metabolism of sphingolipids, but interruption of this metabolism process leads to different diseases.

Glycoconjugate vaccines are composed of microbial poly- or oligosaccharides covalently linked to a carrier protein.

Promotor: G. A. van der Marel, Co-promotor: D. V. Filippov

The work in this thesis has been focused on two subjects. The first is the assembly of alginate oligosaccharides and the generation of building blocks for the enzymatic synthesis of alginate, and the second is the total synthesis of large fragments of the zwitterionic SP1 polysaccharide.

Antibiotic resistance, caused by widespread use of antibiotics, leads to bacterial infections that are difficult, if not impossible, to treat and is a major worldwide health concern.

This thesis describes the design and synthesis of fragments of various cell wall carbohydrates of the Streptococcus species, including the branched Group B-specific antigen (GBC) of Group B Streptococcus, glycerol phosphate (GroP) modified group A carbohydrate (GAC), and the O-acetylated type 1 capsular…

This Thesis describes the synthesis of D-alanylated ribitol-based wall teichoic acids from S. aureus to probe their interaction with immune-binding partners.

The work described in this Thesis is focused on the assembly of oligosaccharide fragments derived from a fungal polysaccharide, galactosaminogalactan (GAG) and fragments of the exopolysaccharide Pel, generated by Pseudomonas aeruginosa.

This thesis investigates catalytic reactions at the atomic scale using scanning tunneling microscopy (STM), with a focus on Fischer–Tropsch synthesis, a key industrial process that converts hydrogen and carbon monoxide into liquid fuels.

Promotor: G.A. van der Marel, Co-promotor: J.D.C. Codée

In 2018, the number of mobile phone users will reach about 4.9 billion. Assuming an average of 5 photos taken per day using the built-in cameras would result in about 9 trillion photos annually.

This thesis describes the design, synthesis, and immunological evaluation of varying (neo)antigenic peptide conjugates containing either a TLR2 or a TLR7 agonist.

This thesis describes the design and synthesis of novel small molecules based on sulfur-containing heterocycles for the inhibition of glycosidases and glycosyltransferases related to Fabry and Pompe disease.

Carbohydrates are present on the surface of bacteria making them suitable antigen candidates for vaccine development. This thesis deals with the synthesis of two carbohydrate-based components; the capsular polisaccharide of S. aureus type 5 and teichoic acids from staphilococci and enterococci speci…

These syntheses allow the team to determine the extent to which schools-based interventions are effective at targeting radicalisation-related outcomes.

This Thesis describes a strategy to synthesize 1,2-cis-oligosaccharides modulated by additives, such as dimethylformamide (DMF), triphenylphosphine oxide (Ph3P=O) and N-methyl(phenyl)formamide (MPF), using building blocks that carry solely benzyl type protecting groups and are therefore of uniform r…

Hydrocarbonylation of alkenes with carboxylic acids in synthesis of carboxylic acid anhydrides is relatively less explored. We herein present a study and optimization of a palladium-catalyzed hydrocarbonylation reaction of alkenes using carboxylic acids as the nucleophile, by which acid anhydrides can…

This thesis comprises the development of reproducible synthesis procedures for two-dimensional materials (composed of boron, nitrogen and carbon) on different metallic surfaces.

Design and synthesis of two 3-substituted swainsonine derivatives with the aim to improve the potency and selectivity towards Golgi alpha-mannosidase II.

This dissertation presents the synthesis and evaluation of antibody recognition for various capsular polysaccharide (CP) fragments of Staphylococcus aureus (S. aureus).

This thesis advances our understanding of lipid uptake, a vital first step in lipid metabolism, by developing innovative click chemistry-based tools to study how immune cells internalize lipids.

The main subject of this thesis is the design and synthesis of paramagnetic molecules for protein studies with NMR and EPR spectroscopy.

The thesis describes the development, synthesis and biological evaluation of several proteasome inhibitor designs. Mainly, this work focusses on designing proteasome inhibitors that selectively inhibit the immunoproteasome, while leaving the constitutive proteasome mostly, if not completely, untouch…

The chemical synthesis of inhibitors and probes targeting endo-glycosidases.

Promotor: H.S. Overkleeft, Co-Promotor: B.I. Florea

Promotores: H.S. Overkleeft, G.A. van der Marel, Co-Promotor: R.G. Boot

Per 1 maart is Nathaniel Martin benoemd tot hoogleraar in Bio-organische chemie aan het Instituut voor Biologie van de Faculteit der Wiskunde en Natuurwetenschappen. In zijn onderzoek zoekt hij nieuwe (bio)chemische benaderingen om besmettelijke ziekten te bestrijden. Ook ontwikkelt hij nieuwe moleculaire…

In de nacht van 2 juli werd chemicus Hermen Overkleeft geïnterviewd in het radioprogramma ’De Nacht Van’ over zijn prijswinnende onderzoek naar aanleiding van zijn toegekende Spinozapremie.

Neuraminidases are enzymes that cleave glycosidic linkages of sialic acid. These enzymes are involved in influenza infections as well as in many cellular processes in mammals and micro-organisms.

Glycoside hydrolases (glycosidases/GHs) are widely abundant enzymes in all kingdoms of life and are important biocatalysts that catalyze the hydrolysis of glycosidic linkages in oligo/polysaccharides, glycoproteins and glycolipids with tremendous efficiency

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt

This thesis describes the synthesis and biochemical evaluation of a variety of cyclophellitol based activity-based probes and inhibitors targeting various endo- and exo-acting retaining glycosidases. In the last two decades a variety of probes and inhibitors for (hemi)cellulose degrading enzymes have…