This thesis describes biochemical investigations of glucocerebrosidase (GBA), the lysosomal β- glucosidase that is deficient in Gaucher disease (GD).

This dissertation revolves around the design and implementation of novel instrumentation and related measurement techniques, at the single molecule level, for use in biophysical research. Chapter 1 presents an introduction to the field of fluorescence-based single molecule measurements. In particular,…

Cardiovascular disease is the leading cause of death worldwide.

Understanding DNA repair mechanisms is essential for advancing plant biotechnology and crop improvement. This study investigates how double-strand breaks (DSBs), induced by CRISPR/Cas systems, are repaired in the model plant Arabidopsis thaliana, and how these processes influence Agrobacterium tumefaciens…

Promotores: Prof.dr. E.A.L. Biessen, Prof.dr. T.J.C. van Berkel

Promotores: Prof.dr. J.N. Kok, Prof.dr. M.R.V. Chaudron (Chalmers Univ., Sweden)

G protein-coupled receptors (GPCRs), one of the largest families of membrane proteins, are responsive to a diverse set of physiological endogenous ligands including hormones and neurotransmitters.

One of the effects of climate change is the phenomenon of desertification, a process that occurs in semi-arid and arid areas and causes land degradation as well as vegetation loss. Due to the lack of resources, vegetation self-organizes to sustain itself by forming large-scale spatial patterns.

Promotor: Prof.dr. D. Bouwmeester

We study the interplay of topology and geometry with chirality for several passive and active systems, employing both analytical and numerical methods.

Acute cardiovascular syndromes, including myocardial infarction or stroke, are the principal cause of death in the Western society. The main underlying pathology of cardiovascular diseases is atherosclerosis, which is caused by the accumulation of lipids and inflammatory cells in the vessel wall, in…

Promotor: J. Zaanen

The skin is our natural barrier and lipids are a key part of this barrier. In the outer skin layer, the stratum corneum (SC), lipids form a densely organized structure dependent on the composition of these lipids.

Continuous optimization is never easy: the exact solution is always a luxury demand and the theory of it is not always analytical and elegant.

In this Ph.D. thesis, we characterized two novel upstream regulators of JUB1, HB40, and HB22, in Arabidopsis thaliana. In Chapter 2, we described functions of HB40 in modulating growth and development via the regulation of JUB1 and GA inactivation genes GA2OXs.

Molecular materials have been a subject of interest in fundamental research and applications for decades, and have been studied as bulk crystals, (thin) films and as individual molecules, due to the large variety in their properties. This dissertation explores pentacene crystals near the two-dimensional…

In this thesis, we have studied the potential of the zebrafish larval model in studying the ECS, as a complementary model to the existing rodent models.

Interactive exploration of large volumes of data is increasingly common, as data scientists attempt to extract interesting information from large opaque data sets. This scenario presents a difficult challenge for traditional database systems, as (1) nothing is known about the query workload in advance,…

Solute carrier (SLC) transporters are a large and diverse class of relatively understudied transmembrane proteins.

Systems are called real-time systems, if the correctness of the system does not only depend on the correctness of the system output but also on whether the output is delivered on time.

Promotor: Prof.dr. T. Hankemeier, Co-promotor: Theo Reijmers

This thesis aims to investigate the effect of tripartite interaction between microbial inoculants, the plant, and herbivore insects on the rhizosphere microbiome and volatilome. We investigated the rhizosphere microbiome and volatilome of tomato plants exposed to insect herbivory and/or inoculated with…

Promotor: J.N. Kok, Co-Promotor: A.J. Knobbe

This thesis investigates reactive interfaces in surface science across three domains: heterogeneous catalysis, environmental nanoplastics, and two-dimensional materials.

Kinase inhibitors are key therapeutic agents, particularly in oncology, yet their clinical efficacy is often hampered by off-target effects and limited understanding of their cellular target profiles.

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We volgen de adellijke dames op hun reizen door Europa, tijdens jachtpartijen, maar ook wanneer ze vertwijfeld op zoek zijn naar geld om hun schulden te betalen. Hoe gaan zij om met de speelruimte die hun wordt toebedeeld? Schikken zij zich in een dienstbare rol of zien ze kans om een eigen kleur te…

Twee documenten markeren de geboorte van de Universiteit Leiden.

Hoe kreeg de politieke cultuur in Vlaanderen en Nederland vorm? Waar liggen de onderlinge verschillen? En hoe gaan beide gebieden daarmee om? Dat lees je in De Lage Landen. Een geschiedenis voor vandaag, een nieuwe uitgave van Ons Erfdeel vzw.

Geavanceerde scans en op maat 3D-geprinte kunstorganen. Medische technologie is niet meer weg te denken uit de zorg. Deze innovatieve behandelmethoden vragen echter om een nieuw soort medisch professional. Iemand met medische én technische kennis, die de brug slaat tussen de techniek en de patiënt.

A single self-assembled semiconductor quantum dot in a high-finesse optical microcavity - the subject of this thesis - is an interesting quantum-mechanical system for future quantum applications. For instance, this system allows trapping of an extra electron and thus can serve as a spin quantum memory,…

Promotores: Prof.dr. P.C.W. Hogendoorn & Prof.dr. H.P. Spaink Co-promotor: Dr. B.E. Snaar-Jagalska

Microbial rhodopsins are photosensitive pigments implemented in the growth and adaptation of a large population of microorganisms.

Promotores: Prof.dr. T.H.W. Bäck, Prof.dr. M.R.V. Chaudron, Co-Promotor: M.T.M. Emmerich

This thesis systematically studies the physicochemical properties of non-planar, propeller-shaped, polycyclic aromatic hydrocarbons. The synthesis of several so-called propellerenes is described on a gram scale, using optimized procedures aimed at using less hazardous reagents and reducing the amount…

Computers worden steeds krachtiger en krijgen steeds ingewikkelder taken. Het Leiden Institute of Advanced Computer Science (LIACS) doet baanbrekend wetenschappelijk onderzoek en past nieuwe vindingen toe in actuele maatschappelijke vraagstukken.

To this day, all cyclophellitol-based inhibitors and ABPs have been close analogues of their natural substrate counterparts. As a result, these probes showed high selectivity towards their target glycosidases.

In this thesis, a system pharmacology approach, integrating metabolomics, pharmacology and chemical biology, was applied to understand and modulate the endocannabinoid system across different model systems (cells, zebrafish, mice and humans).

In this thesis, mathematical modeling and simulation was applied as a tool to inform quantitative decision making in oncology drug discovery and development.

In this thesis, we used genetically engineered mouse models to identify genes and pathways that are involved in ILC formation and in the development of resistance to FGFR-targeted therapy.

In the last decades, activity-based protein profiling (ABPP) has emerged as a powerful chemical tool that may aid the ever-challenging drug discovery process.

Promotor: Prof.dr. M. Ubbink

A difficulty of studies on chemical kinetics are the reaction time scales and detection of their intermediates.