We investigate how radiation pressure can be used to influence the mechanical motion of a micro-mirror suspended from springs.

Ariadne Schmidt, Isabelle Devos and Julie de Groot provide you with refreshing insights concerning the study on urban singles in the period between 1200 and 1900.

Spectroscopic studies on fluorescent single molecules in organic condensed matter does not only provide information about the molecule itself, but also its near environment. By suppression of phonon-induced broadening of spectral lines through cooling to low temperatures, small changes in the spectral…

Surface acoustic waves (SAWs) are mechanical waves that travel along the surface of a material and find many applications in modern technologies due to the ease of excitation on piezoelectric substrates via interdigital transducers (IDTs).

The single cell group (SCG) is part of the Metabolomics & Analytics Centre Our research is centered around developing innovating analytical methods tailored for small-volume, or single cell analysis to enable metabolomics-driven systems biology in personalized health strategies. We are always on…

On 14 December, Jouke Tegelaar defended the thesis 'Single supervision, single judicial protection? Towards effective judicial protection in Single Supervisory Mechanism composite procedures'. The doctoral research was supervised by Matthias Haentjens and Willemien den Ouden.

Single-Molecule Microscopy (SMM) techniques constitute a group of powerful imaging tools that enable researchers to study the dynamic behavior of individual molecules.

In this thesis we investigated the ability of two-photon multifocal microscopy for single-molecule microscopy in live cells and organisms.

Detecting nanoscopic objects plays an important role in nanoscience in particular, in the rapidly growing field of nanobiology. The forebear to modern super-resolution microscopy for single molecule investigation, is fluorescence microscopy.

Single-molecule spectroscopy has become a powerful method for using organic fluorescent molecules in numerous applications.

The sequence-dependence of biomolecular interactions involving nucleic acids and proteins is essential for numerous processes inside the cell. Insights into the underlying molecular mechanisms have been obtained using various biochemical and biophysical methods on two different levels — bulk and sin…

John van Noort

This thesis is a collection of experimental attempts to enhance photoluminescence of fluorescent molecules and quantum dots with single gold nanorods (GNRs) and relevant applications.

The directorate of Leiden Observatory consists of the Scientific Director, the Director of Education and the Director of Operations.

Since the early 1990s, one can isolate the optical signal of a single molecule and single-molecule spectroscopy has quickly grown into an important research field.

Mechanobiology is a major area in biology, however, mechanics is currently a missing element in drug development pipelines. This has led to a high cost for the society. Mechanotoxicity of drugs often appears as a long-term side effect of drugs, and has led to removal of drugs from the market. Furthermore,…

In this work, we investigate the minute circular dichroism effects of single nanoparticles.To this aim, we apply photothermal imaging with a polarization-modulated heating beam.

Promotores: Prof.dr. D. Bouwmeester, Prof.dr. A. Fiore (TU Eindhoven)

The goal is to reduce single use coffee cup use both at the university cafés and cofee machines.

In this thesis, we study different curved single crystals because of the diversity of surface structures across their curvature.

Intrigued by the way cells autonomously regulate their fate, we strive to understand and visualize cellular processes as the basis of Cell Signaling.

This dissertation revolves around the design and implementation of novel instrumentation and related measurement techniques, at the single molecule level, for use in biophysical research. Chapter 1 presents an introduction to the field of fluorescence-based single molecule measurements. In particular,…

An Ancient Egyptian System of Workmen’s Identity Marks

Visualization of biomolecules in living cells by tagging with gold nanoparticles.

Promotor: Prof.dr. T. Schmidt, Co-promotor: Dr. ir. S. J.T. van Noort

We study cell-fate decision-making using embryonic stem cells as a model system. Stem cells integrate a large number of cues to direct their development into a great variety of cell types.

Promotores: Prof.dr. G.W. Canters, Prof.dr. T.J. Aartsma

Promotor: Prof.dr. M.A.G.J. Orrit

Curved single crystals provide variable, but well-defined surface structures.

Promotor: Prof.dr. T. Hankemeier, Co-promotores: Heiko van der Linden, Paul Vulto

The use-wear analysis of domestic implements provided new insights of the Single Grave Culture population in the Netherlands.

In human cells, a meter-long DNA is condensed inside a micrometer-sized cell nucleus. Simultaneously, the genetic code must remain accessible for its replication and transcription to functional proteins.

In this thesis, we describe the potential of cell mechanical phenotyping for immune cell characterization, diagnosis, drug testing, as well as treatment of disease.

Advances in the field of high-performance Nd-Fe-B permanent magnets, by re-engineering their microstructure to make them more powerful while reducing rare earth content.

This thesis aims to improve the detection from ultra-weak single emitter by enhancing their emission properties with plasmonic nanostructures. We exploit the wet-chemically synthesized single crystalline gold nanorods (GNRs) as our basic frameworks in the whole studies, simply because of their unique…

A single self-assembled semiconductor quantum dot in a high-finesse optical microcavity - the subject of this thesis - is an interesting quantum-mechanical system for future quantum applications. For instance, this system allows trapping of an extra electron and thus can serve as a spin quantum memory,…

Early time-resolved experiments by Frauenfelder on the ensemble of the kinetic rebinding of CO to myoglobin molecules resulted in a stretched exponential relaxa-tion due to a very large spread of the reaction rates of individual molecules.

Gold nanorods are biocompatible nanoparticles that present an excellent two-photon signal that can be used to get high spatial resolution inside living cells. Gold nanorods are photostable and therefore can be followed inside cells for long time, with possible applications as trackers in live cells.

Promotor: M.A.G.J. Orrit

Can we find new chemical and biological sensing routes on the edge and surface of graphene to improve the potential of graphene to act as a sensor?

Microneedles represent promising tools for delivery of drugs to the skin. However, before these microneedles can be used in clinical practice, it is essential to understand the process of skin penetration by these microneedles.

Acute cardiovascular syndromes, including myocardial infarction or stroke, are the principal cause of death in the Western society. The main underlying pathology of cardiovascular diseases is atherosclerosis, which is caused by the accumulation of lipids and inflammatory cells in the vessel wall, in…

Science, Leiden Academic Centre for Drug Research (LACDR)

In Quantum mechanics, particles can be in multiple positions simultaneously. Yet, when a measurement is made, the particle is found only in one place. Technology has come to a point where we may design experiments that will tell us how.

This thesis consists of two main parts. The first part (Chapters 2 and 3) deals with a class of Markov process called Quasi-Skipfree (QSF) processes.

The deeper understanding of an organism's pathology is important for developing treatments. Over centuries of systematic research, clinical researchers have demonstrated that the more information they acquire about the cellular properties and their organisation in the tissue, the better they can understand…

Despite being the object of intense study, embryonic development has been difficult to model due to a number of reasons. First, complex tissues can be comprised of many cell types, of which we probably only know a subset.

Optical detection of a single electron using a single molecule has never been done. Leiden physicist Michel Orrit and his team have now identified a molecule that is sensitive enough to detect an electron at a distance of hundreds of nanometers. The results are published as a cover article in ChemPh…