Streptomyces are multicellular, Gram-positive bacteria in the phylum of actinobacteria which produce a high amount of bioactive natural products of which the expression is tightly coordinated with the life cycle.

The loss of T-cell homeostasis and tolerance towards self-antigens is the underlying cause of autoimmune and inflammatory diseases like atherosclerosis, rheumatoid arthritis or type I diabetes. Traditionally, the treatment of these diseases consisted on systemic immune suppression, which can entail…

Chemist Roxanne Kieltyka has received an ERC Starting Grant of 2 million euros. In her lab, she creates gels that mimic the instructive material that supports cells in our body. With the grant, she aims to make these gels stiff and tough, and to create a bio-printed miniature heart ventricle.

In our research protein translocation from Agrobacterium into yeast and plant cells is studied to obtain fundamental insights in the translocation process

One of the major limitations in culturing complex tissues or organs is the lack of vascularization in the cultured tissue. Development of a functional capillary bed could overcome this problem.

Promotor: Prof.dr. R. Verpoorte, Co-Promotores: N.R. Mustafa, A.E. Schulte

Promotor: C.A.J. Knibbe, Co-promotor: J.J. Boelens, R.G.M. Bredius

Promotores: Prof.dr. E.A.L. Biessen, Prof.dr. T.J.C. van Berkel

The deeper understanding of an organism's pathology is important for developing treatments. Over centuries of systematic research, clinical researchers have demonstrated that the more information they acquire about the cellular properties and their organisation in the tissue, the better they can understand…

In this thesis, the focus lies on studying glucocorticoid receptor dynamics in living cells with the aim of understanding how this transcription factor finds its DNA target sites to regulate transcription.

School pupils are all different, which is why there is differentiation in our education system. This can be seen in pre-university education and lower vocational schools, and also takes the form of ability groups in junior schools. But according to Professor by Special Appointment Eddie Denessen, differentiation…

Promotor: A.H. Meijer, Co-promotor: B.E. Snaar-Jagalska

Across Europe, new welfare programs exemplify attempts to govern through community. This article asks how such governing through community is done in practice. Drawing on comparative insights from fieldwork with parenting support professionals and volunteers in Amsterdam, Milan, and Paris, we document…

This is an Erasmus+ International Credit Mobility project of Leiden University’s Faculty of Medicine with The Wistar Institute in the United States.

The lipid matrix of the skin's stratum corneum plays a key role in the barrier function, which protects the body from desiccation. The lipids that make up this matrix consist of ceramides, cholesterol, and free fatty acids, and can form two coexisting crystalline lamellar phases: the long periodicity…

This thesis describes the antimicrobial discovery strategy developed in our group, the den Hertog Group at the Hubrecht Institute. It includes a cultivation-based screening approach for novel antimicrobial agents from the source of fungi, and a bacterial time-lapse imaging approach for antimicrobial…

Light-Harvesting Complex II (LHCII) is responsible for light absorption and excitation energy transfer in plants and photosynthetic algae, while in high light it undergoes conformational changes by which it quenches excitations to prevent photodamage.

This thesis focused on the exploration of monolayer graphene as a membrane in direct methanol fuel cell (DMFC) applications, with improvements achieved through plasma exposure or damage- free chemical functionalization.

Dendritic cells (DC) play a prominent role in the priming of CD8(+) T cells. Vaccination is a promising treatment to boost tumor-specific CD8(+) T cells which is crucially dependent on adequate delivery of the vaccine to DC.

Cells in our body are constantly performing small tasks, such as repairing wounds. They exert force by changing shape. But how do cells translate their shape into exerting a force in a specific direction? Experimental and theoretical Leiden physicists have now found a clue to answer this question. Cells’…

The aim of this work was to study the applicability of antigen-coated pH-sensitive microneedle arrays for effective vaccination strategies. Therefore, a model antigen (ovalbumin) was coated onto pH-sensitive (pyridine-modified) microneedle arrays to test pH-triggered antigen release by applying the…

This collection of essays is the first to compare the emergence and development of these different types of regional identities.

The emergence of complex diseases resulting from abnormal cell-cell signaling and the spread of infectious diseases caused by pathogens are significant threats to humanity. Unraveling the dynamic mechanisms underlying cell-cell signaling and infectious disease spreading is crucial for effective disease…

This book explores how public health concerns and political agendas influenced each other in the US over the past century.

On 17 April, Evert-Jan Govaers defended the thesis 'Ministers of State. Unknown and desired; from stage to the wings?'.The doctoral research was supervised by Wim Voermans.

In this project of the Center for Public Values & Ethics (CPVE) ongoing research is conducted on the history of advice to Dutch central government by so-called State committees from 1814 to 2014. State committees have been installed by Dutch central government since 1814 to provide expert advice, research…

Managing scarcity to serve the public interest is a classic government task. An important way to execute this task is by allocating individual rights that are only available in limited quantities, such as CO2 emission allowances, gambling licences, subsidies, radio frequencies, public contracts and…

This article provides a comprehensive analysis of the Islamic State's use of images taken by drones, drawing on a dataset of ISIS propaganda images from October 2016 to December 2018.

Poly(lactic-co-glycolic acid) (PLGA) particles have been extensively studied as biodegradable delivery system to improve the potency and safety of protein-based vaccines. In this study we analyzed how the size of PLGA particles, and hence their ability to be engulfed by dendritic cells (DC), affects…

This is an Erasmus+ International Credit Mobility project of Leiden University’s Faculty of Science with University of California.

Cell-based medicinal products (CBMPs) show great promise when it comes to the treatment of genetic disorders. However, there is limited knowledge about CBMP stability during their production, storage, and administration to patients. The main focus of my research is the characterization of CBMPs using…

A very large part of intravenously administered nanoparticles are cleared through the liver. Within the liver, most nanoparticles are thought to be sequestered by macrophages (Kupffer cells). To achieve effective cell-specific targeting of drugs and non-viral gene delivery vectors, improved mechanistic…

This thesis describes the development of a variety of mannosylated conjugates. Antigen presenting cells bear mannoside recognizing receptors that actively transport antigen into the cell. This thesis exploits this feature for the development of improved vaccines.

PhD defence

PhD defence

State Building Through Life Stories: Incorporating Local Perspectives

For effective cancer immunotherapy by vaccination, co-delivery of tumour antigens and adjuvants to dendritic cells and subsequent activation of antigen-specific cytotoxic T cells (CTLs) is crucial. In this study, a synthetic long peptide (SLP) harbouring the model CTL epitope SIINFEKL was encapsulated…

This edited volume by Jeff Fynn-Paul pushes forward the debate on the role of entrepreneurs in making war and building states in Europe and the Ottoman Empire.

Promotor: Prof.dr. H.P. Spaink Co-promotor: Dr. B.E. Snaar

How does an embryonic stem cell decide if it becomes a heart cell or a kidney cell? That’s the question computational biologist Maria Mircea studied for her PhD research. She looked at the inside of individual cells to analyse how they change. This is what she discovered.

This dissertation is organised into three parts, each addressing a distinct research area related to Gibbs measures.

The States, Markets, and Civil Society department of the Institute of Public Administration examines how politics and governance, businesses, citizens, and societal organisations collaborate to address major societal challenges. This includes issues such as inequality, migration, and sustainability.

Thread-like bacteria make cells that no longer have a cell wall under the influence of osmotic stress. A remarkable discovery, since the cell wall serves as a protection barrier for bacteria. It could also help to explain how pathogenic bacteria can hide in our body from our immune system. A team of…

Promotor: W.Th.F. den Hollander Co-Promotor: F.M. Spieksma

The Europa Instituut is pleased to announce that on 21 November 2016 a new handbook “EU State Aids” (31 Chapters, 1500 pages) was published.